Open Access Open Access  Restricted Access Subscription Access

A study on bacteria associated with three hard coral species from Ninh Thuan waters by epifluorescence and most diluted culture method

Pham Thi Mien, Nguyen Kim Hanh, Nguyen Minh Hieu, Phan Minh Thu, Hoang Trung Du, Vo Hai Thi, Nguyen Trinh Duc Hieu, Le Tran Dung, Nguyen Huu Huan

Abstract


Coral associated bacteria and their host are currently one of the interested issues for research and scientists worldwide. The densities of zooxanthellae and bacteria associated with three most prevalent species Acropora hyacinthus, Acropora muricata and Acropora robusta in Hang Rai, Ninh Thuan was evaluated over time by staining with SYBR Gold and direct counting with epifluorescence method. The most dominant bacteria were isolated by culture dependent method. The densities of zooxanthellae and bacteria ranged from 0.39–1.83×107 cell/g, and 0.83–2.52×108 cell/g, respectively. Bacterial density in the 3 months was significantly different compared to the density of the bacteria in ambient water. Total heterotrophic bacteria, comma shaped bacteria and bacillus form showed negatively correlated with pH, PO4, while zooxanthellae showed no correlation with all factors.



Keywords


Symbiotic microbes, bacteria, Acropora hyacinthus, Acropora muricata, Acropora robusta, environmental parameters, Ninh Thuan.

Full Text:

PDF

References


Vo, S. T., Pernetta, J. C., and Paterson, C. J., 2013. Status and trends in coastal habitats of the South China Sea. Ocean & Coastal Management, 85, 153–163.

Võ Sĩ Tuấn, Lyndon DeVantier, Nguyễn Văn Long, Hứa Thái Tuyến, Nguyễn Xuân Hòa, và Phan Kim Hòang, 2002. Nghiên cứu thành phần loài, cấu trúc quần xã và hiện trạng rạn san hô nhằm đề xuất giải pháp quản lý đa dạng sinh học ở khu bảo tồn biển Hòn Mun, vịnh Nha Trang. Hội nghị khoa học “Biển Đông-2002”. Nxb. Nông nghiệp. Tr. 649–690.

Tuan, V. S., 2002. Report on status of coral reefs in Vietnam: 2000. In Proceedings of the Ninth International Coral Reef Symposium, Bali, 23–27 October 2000, (Vol. 2, pp. 891–899).

Bell, J. J., Davy, S. K., Jones, T., Taylor, M. W., and Webster, N. S., 2013. Could some coral reefs become sponge reefs as our climate changes?. Global Change Biology, 19(9), 2613–2624.

Carballo, J. L., Bautista, E., Nava, H., Cruz‐Barraza, J. A., and Chávez, J. A., 2013. Boring sponges, an increasing threat for coral reefs affected by bleaching events. Ecology and Evolution, 3(4), 872–886.

Tun, K., Chou, L. M., Low, J., Yeemin, T., Phongsuwan, N., Setiasih, N.,Wilson, J., Amri, A. Y., Adzis, K. A. A., Lane, D., Bochove, J-W. V., Kluskens, B., Nguyen, V. L., Vo, S. T., and Gomez, E., 2010. Regional overview on the 2010 coral bleaching event in Southeast Asia. In: Status of Coral Reefs in East Asian Seas Regions: 2010. Ministry of the Environment of Japan, 9–27.

Rowan, R., Knowlton, N., Baker, A., and Jara, J., 1997. Landscape ecology of algal symbionts creates variation in episodes of coral bleaching. Nature, 388(6639), 265–269.

Rosenberg, E., Kushmaro, A., Kramarsky-Winter, E., Banin, E., and Yossi, L., 2009. The role of microorganisms in coral bleaching. The ISME Journal, 3(2), 139–146.

Rosenberg, E., Koren, O., Reshef, L., Efrony, R., and Zilber-Rosenberg, I., 2007. The role of microorganisms in coral health, disease and evolution. Nature Reviews Microbiology, 5(5), 355–362.

Kvennefors, E. C. E., Sampayo, E., Kerr, C., Vieira, G., Roff, G., and Barnes, A. C., 2012. Regulation of bacterial communities through antimicrobial activity by the coral holobiont. Microbial Ecology, 63(3), 605– 618.

Shnit-Orland, M., Sivan, A., and Kushmaro, A., 2012. Antibacterial activity of Pseudoalteromonas in the coral holobiont. Microbial Ecology, 64(4), 851–859.

Ritchie, K. B., 2006. Regulation of microbial populations by coral surface mucus and mucus-associated bacteria. Marine Ecology Progress Series, 322, 1–14.

Raina, J. B., Dinsdale, E. A., Willis, B. L., and Bourne, D. G., 2010. Do the organic sulfur compounds DMSP and DMS drive coral microbial associations?. Trends in Microbiology, 18(3), 101–108.

Raina, J. B., Tapiolas, D., Willis, B. L., and Bourne, D. G., 2009. Coral-associated bacteria and their role in the biogeochemical cycling of sulfur. Applied and Environmental Microbiology, 75(11), 3492–3501.

Bourne, D., Iida, Y., Uthicke, S., and Smith-Keune, C., 2008. Changes in coral-associated microbial communities during a bleaching event. The ISME Journal, 2(4), 350–363.

Garren, M., Son, K., Raina, J. B., Rusconi, R., Menolascina, F., Shapiro, O. H., Tout, J., Bourne, D. G., Seymour, J. R., and Stocker, R. (2014). A bacterial pathogen uses dimethylsulfoniopropionate as a cue to target heat-stressed corals. The ISME journal, 8(5), 999–1007.

Raina, J. B., Tapiolas, D., Motti, C. A., Foret, S., Seemann, T., Tebben, J., Willis, B. L., and Bourne, D. G., 2016. Isolation of an antimicrobial compound produced by bacteria associated with reef-building corals. PeerJ, 4, e2275.

Baird, R. B., Eaton, A. D., and Clesceri, L. S., 2012. Standard methods for the examination of water and wastewater (Vol. 10). E. W. Rice (Ed.). Washington, DC: American Public Health Association.

Leruste, A., Bouvier, T., and Bettarel, Y., 2012. Enumerating viruses in coral mucus. Applied and Environmental Microbiology, 78(17), 6377–6379.

Team, R. C., 2013. R: A language and environment for statistical computing.

Lien, Y. T., Fukami, H., and Yamashita, Y., 2012. Symbiodinium clade C dominates zooxanthellate corals (Scleractinia) in the temperate region of Japan. Zoological Science, 29(3), 173–181.

Fabricius, K. E., Mieog, J. C., Colin, P. L., Idip, D., and van Oppen, M. J., 2004. Identity and diversity of coral endosymbionts (zooxanthellae) from three Palauan reefs with contrasting bleaching, temperature and shading histories. Molecular Ecology, 13(8), 2445–2458.

Silverstein, R. N., Cunning, R., and Baker, A. C., 2015. Change in algal symbiont communities after bleaching, not prior heat exposure, increases heat tolerance of reef corals. Global change biology, 21(1), 236–249.

Bellantuono, A. J., Hoegh-Guldberg, O., and Rodriguez-Lanetty, M., 2011. Resistance to thermal stress in corals without changes in symbiont composition. Proceedings of the Royal Society B: Biological Sciences, 279(1731), 1100–1107.

Moore, R. B., Oborník, M., Janouškovec, J., Chrudimský, T., Vancová, M., Green, D. H., Wright, S. W., Davies, N. W., Bolch, C. J. S., Heimann, K., Šlapeta, J., Hoegh-Guldberg, O., Logsdon, J. M., and Carter, D. A., 2008. A photosynthetic alveolate closely related to apicomplexan parasites. Nature, 451(7181), 959–963.

Cumbo, V. R., Baird, A. H., Moore, R. B., Negri, A. P., Neilan, B. A., Salih, A., van Oppen, J. H., Wang, Y., and Marquis, C. P., 2013. Chromera velia is endosymbiotic in larvae of the reef corals Acropora digitifera and A. tenuis. Protist, 164(2), 237–244.

Hanh, N. K., Bettarel, Y., Bouvier, T., Bouvier, C., Hai, D. N., Lam, N. N., Thuy, N. T., Huy, T. Q., and Brune, J., 2015. Coral mucus is a hot spot for viral infections. Applied and Environmental Microbiology, 81(17), 5773–5783.

Nguyen‐Kim, H., Bouvier, T., Bouvier, C., Doan‐Nhu, H., Nguyen‐Ngoc, L., Rochelle‐Newall, E., Baudoux, A. C., Desnues, C., Reynaud, S., Ferrier‐Pages, C., and Bettarel, Y., 2014. High occurrence of viruses in the mucus layer of scleractinian corals. Environmental Microbiology Reports, 6(6), 675–682.

Phạm Thị Miền, Võ Hải Thi, Lê Hoài Hương và Hoàng Xuân Bền, 2010. Phân lập vi khuẩn từ san hô mềm Sinularia spp. và thử nghiệm hoạt tính kháng Tetracycline, Gentamicin, Cefazolin của chúng. Tuyển tập nghiên cứu biển, 17, 183–195.

Pham, T. M., Wiese, J., Wenzel-Storjohann, A., and Imhoff, J. F., 2016. Diversity and antimicrobial potential of bacterial isolates associated with the soft coral Alcyonium digitatum from the Baltic Sea. Antonie Van Leeuwenhoek, 109(1), 105–119.

Meron, D., Atias, E., Kruh, L. I., Elifantz, H., Minz, D., Fine, M., and Banin, E., 2011. The impact of reduced pH on the microbial community of the coral Acropora eurystoma. The ISME Journal, 5(1), 51–60.

Meron, D., Rodolfo-Metalpa, R., Cunning, R., Baker, A. C., Fine, M., and Banin, E., 2012. Changes in coral microbial communities in response to a natural pH gradient. The ISME Journal, 6(9), 1775–1785.

Simon, C., and Daniel, R., 2011. Metagenomic Analyses: Past and Future Trends. Applied and Environmental Microbiology, 77(4), 1153–1161.

Nithyanand, P., and Pandian, S. K., 2009. Phylogenetic characterization of culturable bacterial diversity associated with the mucus and tissue of the coral Acropora digitifera from the Gulf of Mannar. FEMS Microbiology Ecology, 69(3), 384–394.

de Castro, A. P., Araújo, S. D., Reis, A. M., Moura, R. L., Francini-Filho, R. B., Pappas, G., Rodrigues, T. B., Thompson, F. L., and Krüger, R. H., 2010. Bacterial community associated with healthy and diseased reef coral Mussismilia hispida from eastern Brazil. Microbial Ecology, 59(4), 658–667.

Rachanamol, R. S., Lipton, A. P., Thankamani, V., Sarika, A. R., and Selvin, J., 2014. Molecular characterization and bioactivity profile of the tropical sponge-associated bacterium Shewanella algae VCDB. Helgoland marine research, 68(2), 263–269.

Yazdani, M., Bahmanyar, M. A., Pirdashti, H., & Esmaili, M. A. (2009). Effect of phosphate solubilization microorganisms (PSM) and plant growth promoting rhizobacteria (PGPR) on yield and yield components of corn (Zea mays L.). World Academy of Science, Engineering and Technology, 49, 90–92.

Gyaneshwar, P., Parekh, L. J., Archana, G., Poole, P. S., Collins, M. D., Hutson, R. A., and Kumar, G. N., 1999. Involvement of a phosphate starvation inducible glucose dehydrogenase in soil phosphate solubilization by Enterobacter asburiae. FEMS microbiology letters, 171(2), 223–229.




DOI: https://doi.org/10.15625/1859-3097/19/2/10814 Display counter: Abstract : 19 views. PDF : 4 views.

Journal of Marine Science and Technology ISSN: 1859 3097

Published by Vietnam Academy of Science and Technology