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ANTIBIOTIC RESISTANCES OF MARINE BACTERIA FROM HON MOT, NHA TRANG BAY

Pham Thi Mien, Dao Viet Ha, Nguyen Kim Hanh

Abstract


Drug resistance is now an issue of deep scientific concern all over the world as more antibiotic resistant bacteria have been detected in many regions and countries in recent years. Using of antibiotics for aquaculture is quite common in developing countries including Vietnam. This causes the spread of antibiotic resistant bacteria in the environment. Testing of antibiotic resistant bacteria was carried out in commercial marine aquaculture and tourist zones in Hon Mot in the Nha Trang bay with five different antibiotics namely chloramphenicol, tetracycline, cefazolin, streptomycin, and gentamicin on Mueller Hinton Agar. A total of 18 strains composed of 14 Gram positive strains, 4 Gram negative strains were tested for antibiotic resistance. Strain KH1 showed the least sensitive to tetracycline with average radius of 4.0 mm, while strain T1 showed the least sensitive to streptomycin with average radius of 4.2 mm. The strains KH6 and T7 showed resistance to cefazolin with radius of inhibition zone of 0 mm and 2 mm, respectively. The strain KH6 was confirmed as Bacillus sp. Antibiotic resistant bacteria were now found in Nha Trang bay, so there may be additional resistant bacteria, even multi-antibiotic resistant bacteria outside the marine environment. This is a matter for further research that should provide solutions to limit the rise of antibiotic resistant bacteria.

Keywords


Antibiotic resistant bacteria, Hon Mot, Nha Trang.

References


Silbergeld, E. K., Graham, J., and Price, L. B., 2008. Industrial food animal production, antimicrobial resistance, and human health. Annu. Rev. Public Health, 29, 151-169.

Guglielmetti, E., Korhonen, J. M., Heikkinen, J., Morelli, L., and Von Wright, A., 2009. Transfer of plasmid-mediated resistance to tetracycline in pathogenic bacteria from fish and aquaculture environments. FEMS microbiology letters, 293(1), 28-34.

Alderman, D. J., and Hastings, T. S., 1998. Antibiotic use in aquaculture: development of antibiotic resistance-potential for consumer health risks. International Journal of Food Science and Technology, 33(2), 139-155.

Hoa, P. T. P., Managaki, S., Nakada, N., Takada, H., Shimizu, A., Anh, D. H., ... and Suzuki, S., 2011. Antibiotic contamination and occurrence of antibiotic-resistant bacteria in aquatic environments of northern Vietnam. Science of The Total Environment, 409(15), 2894-2901.

Hoa, P. T. P., Nonaka, L., Viet, P. H., and Suzuki, S., 2008. Detection of the sul1, sul2, and sul3 genes in sulfonamide-resistant bacteria from wastewater and shrimp ponds of north Vietnam. Science of The Total Environment, 405(1), 377-384.

Nguyen, H. N. K., Van, T. T. H., Nguyen, H. T., Smooker, P. M., Shimeta, J., and Coloe, P. J., 2014. Molecular characterization of antibiotic resistance in Pseudomonas and Aeromonas isolates from catfish of the Mekong delta, Vietnam. Veterinary Microbiology, 171(3), 397-405.

Akinbowale, O. L., Peng, H., and Barton, M. D., 2006. Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. Journal of Applied Microbiology, 100(5), 1103-1113.

Halebian, S., Harris, B., Finegold, S. M., and Rolfe, R. D., 1981. Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria. Journal of Clinical Microbiology, 13(3), 444-448.

Bergey’s manual of Systematic Bacteriology. Baltimore, London. (1984). Vol 2.

Bauer, A. W., Kirby, W. M., Sherris, J. C., and Turck, M., 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4), 493-496.

Phạm Thị Miền, Võ Hải Thi, Lê Hoài Hương và Hoàng Xuân Bền. (2010). Phân lập vi khuẩn từ san hô mềm Sinularia spp. và thử nghiệm hoạt tính kháng Tetracycline, Gentamicin, Cefazolin của chúng. Tuyển tập nghiên cứu biển XVII, 183-195.

DCT, R., 2009. R: A language and enviroment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing. ISBN 3-900051-07-0.

CLSI., 2014. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. The Clinical and Laboratory Standards Institute (CLSI) M100-S24, Wayne, PA, USA.

Gao, P., Mao, D., Luo, Y., Wang, L., Xu, B., and Xu, L., 2012. Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment. Water Research, 46(7), 2355-2364.

Akinbowale, O. L., Peng, H., and Barton, M. D., 2007. Diversity of tetracycline resistance genes in bacteria from aquaculture sources in Australia. Journal of Applied Microbiology, 103(5), 2016-2025.

Từ Thanh Dung, Freddy Haesebrouk, Nguyễn Anh Tuấn, Partrick Sorgeloos, Margo Baele và Annemie Decostere, (2010). Hiện trạng kháng thuốc kháng sinh trên vi khuẩn Edwardsiella ictaluri gây bệnh gan, thận mủ trên cá tra (Pangasianodon hypophthalmus) ở đồng bằng sông Cửu Long. Tạp chí khoa học, 15, 162-171.

Wang, R. X., Wang, A., and Wang, J. Y., 2014. Antibiotic resistance monitoring in heterotrophic bacteria from anthropogenic-polluted seawater and the intestines of oyster Crassostrea hongkongensis. Ecotoxicology and Environmental Safety, 109, 27-31.




DOI: https://doi.org/10.15625/1859-3097/17/4/8672 Display counter: Abstract : 59 views. PDF (Tiếng Việt) : 0 views.

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