Changes in glycosylated flavonoids in soybean (Glycine max (L.) Merr. cv. “Nam Dan”) leaves under aphid infestation

Mai Thị Thơm, Trần Ngọc Toàn, Trần Thị Thanh Huyền, Mai Văn Chung

Abstract


Changes in contents of glycosylated flavonoids in leaves of soybean (Glycine max (L.) Merr. cv. “Nam Dan”) in the flowering stage under infestation of cowpea aphid (Aphis craccivora Koch) was investigated. A strong decrease in the level of glycosylated flavonoids, e.g., genistein 7-O-glucoside, 2’OH genistein 7-O-glucoside, 2’OH genistein malonyl glucoside, after 48-96 hours post-infestation (hpi) of aphid. Levels of these compounds in aphid-infested leaves were remarkably lower than those in controls. Whereas, genistein and total flavonoids in the aphid-infested leaves were induced to higher contents than that observed in control. Activity of important enzymes regarding biosynthesis of flavonoids such as phenylalanine ammonia-lyase (PAL, EC. 4.3.1.5), chalcone synthase (CHS, EC 2.3.1.74) and chalcone isomerase (CHI, EC 5.5.1.6) in the aphid-infested leaves strongly increased during experiemntal time. An increase in acitivity of those enzymes was proportional with the induced decrease of the glycosylated flavonoids and an accumulated increase of genistein and total flavonoids. These results indicate that, changes in the glycosylated flavonoids occured in soybean “Nam Dan” leaves infested by cowpea aphid may connect to a protective mechanism in this soybean cultivar in the flowering stage.


Keywords


soybean “Nam Dan”, cowpea aphid, glycosylated flavonoids, enzymes

Full Text:

PDF

References


Samanta A., Das G., Das S. K. - Role of flavonoids in plants, Int. J. Pharm. Sci. Tech. 6 (1) (2011) 12-35.

Ringli C., Bigler L., Kuhn B. M. , Leiber R. M., Diet A., Santelia D., Frey B., Pollmann S., Klein M. - The modified flavonol glycosylation profile in the Arabidopsis rol1 mutants results in alterations in plant growth and cell shape formation, The Plant cell 20 (2008) 1470–1481.

Mai Van Chung, Doan Manh Dung, and Do Ngoc Dai - Accumulation of flavonoids in soybean under effects of Cyanobacteria crude extract and aphid infestation, Journal of Science and Technology (Vietnam), 54 (2C) (2016) 271-277.

Dixon R. A., and Ferreira D. - Genistein, Phytochemistry, 60 (3) (2002) 205-211.

Stobiecki M., Wojtaszek P., and Gulewicz K. - Application of solid phase extraction for profiling of quinolizidine alkaloids and phenolic compounds in lupin tissue, Phytochem. Anal. 8 (1997) 153-158.

Franski R., Bednarek P., Wojtaszek P., Stobiecki M. - Identification of flavonoid diglycosides in yellow lupin (Lupinus luteus L.) with mass spectrometric techniques, J. Mass Spectrom. 34 (1999) 486-495.

Chang C. C., Yang M. H., Wen H. M., and Chern J. C. - Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J. Food Drug Anal. 10 (2002) 178-182.

Cahill D. M., and McComb J.A. - A comparison of changes in phenylalanine ammonialyase activity. Lignin and phenolic synthesis in the roots of Eucalyptus callophylla (field resistant) and E. marginata (susceptible) when infected with Phytophtora cinnamoni, Physiol. Mol. Plant Pathol. 40 (1992) 315–332.

Ghasemzadeh A., Nasiri A., Jaafar H. Z. E., Baghdadi A., and Ahmad I. - Changes in phytochemical synthesis, chalcone synthase activity and pharmaceutical qualities of Sabah snake grass (Clinacanthus nutans L.) in relation to plant age, Molecules 19 (11) (2014) 17632-17648.

Lister C. E., and Lancaster J. E. - Developmental changes in enzymes biosynthesis in the skins of red and of flavonoid green apple cultivars, J. Sci. Food Agric. 71 (1996) 313-330.

Shimoda K., Kubota N., Hamada H., and Hamada H. - Synthesis of gentiooligosaccharides of genistein and glycitein and their radical scavenging and anti-allergic activity, Molecules, 16 (2011) 4740-4747.

Tran Ngoc Toan, Tran Thi Thanh Huyen, Mai Van Chung - Antioxidative defense responses to aphid-induced oxidative stress in Glycine max (L.) Merr. cv. “Nam Dan”. Journal of Science and Technology (Vietnam), 54 (6) (2016) 719-728.




DOI: https://doi.org/10.15625/2525-2518/56/4A/12744

Refbacks

  • There are currently no refbacks.


Index: Google Scholar; Crossref; VCGate; Asean Citation Index

Published by Vietnam Academy of Science and Technology