EFFICIENT STARCH RECOVERY FROM CASSAVA BAGASSE: ROLE OF CELLULASE AND PECTINASE

Pham Tuan Anh, Pham Kim Ngan, Kim Anh To

Abstract


 In this paper, the bagasses were treated with single pectinase (Pectinex Ultra SP-L, Novozymes) and cellulase (NS22192, Novozymes) or their mixture at various enzyme ratio. The response surface methodology was used to fit the experimental data and investigate the enzyme ratio influence on the starch recovery yield.  The highest starch yield was achieved in the treatment of bagasse with 15 CMCU and 9PGU per gram of dry bagasse at 450C and pH 5 for 4 hours at the solid loading 5%, attained  86.55% of the residue starch.


Keywords


Starch recovery, cassava bagasse, cellulase, pectinase

Full Text:

PDF

References


Balagopalh C., Ray R.C., Sheriff J.T., Rajalekshmy L.-Biotechnology for the value addition of waste and residues from cassava processing industries. In: Procceding of the second international sciencetific meeting of the cassava biotechnology network. Bogor, Indonesia, 22-26 Aug (1994) 690-701.

Edama N.A., Sulaiman A., Rahim S.N.A. -Enzymatic saccharification of Tapioca processing wastes into biosugars through immobilization technology (Mini Review). Biofuel Research Journal 1 (1) (2014) 2-

Kallabinski J., Balagopalan C.-Enzymatic starch extraction from tropical root and tuber crops. International Society for Horticultural Science (ISHS), Leuven, Belgium, (1994) 83-88.

Miller G.L.-Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31(3) (1959) 426-428.

Pandey A., Soccol C.R., Nigam P., Soccol V.T., Vandenberghe L.P.S., Mohan R.- Biotechnological potential of agro-industrial residues. II: cassava bagasse. Bioresource Technology 74 (1) (2000) 81-87.

Sluiter A., Hames B., Ruiz R., C Scarlata., Sluiter J., D Templeton., Crocker D.- Determination of structural carbohydrates and lignin in biomass. Technical Report NREL/TP-510-42618. National Renewable Energy Laboratory (2011).

Sriroth K., Chllakup R., Chotineerant S., Piyachomkwan K., Oates C.G.- Processing of cassava waste for improved biomass utiilization. Bioresource Technology 71(2000) 63-69.

Sriroth K., Piyachomkwan K., Wanlapatit S., Oates C.G.-Cassava Starch Technology: The Thai Experience. Starch - Stärke, 52(12) (2000) 439-449.

Woiciechowski A.L., Nitsche S., Pandey A., Soccol C.R.-Acid and enzymatic hydrolysis to recover reducing sugars from cassava bagasse: an economic study. Brazilian Archives of Biology and Technology 45 (2002) 393-400.




DOI: https://doi.org/10.15625/2525-2518/57/4/12764 Display counter: Abstract : 269 views. PDF : 89 views.

Refbacks

  • There are currently no refbacks.


Index: Google Scholar; Crossref; VCGate; Asean Citation Index

Published by Vietnam Academy of Science and Technology