Effects of nutrient medium on Thalassiosira pseudonana’s growth

Cong Van Nguyen, Tran Dinh Quang, Nguyen Kim Duong, Do Thi Hoa Vien

Abstract


Biomass culture of microalga T. pseudonana in F2, TMRL, AGP, Conway and Walne nutrient media. In the AGP nutrient medium, T. pseudonana culture reached a maximum cell density of 1.23 ± 0.04 × 106 cells/ml with a specific growth rate of µ = 0.23/day after 6 days of cultivation. On the Conway, TMRL, Walne and F2 nutrient media, T. pseudonana reached maximum cell densities of 1.03 ± 0.01 × 106 cells/mL, 0.94 ± 0.03 × 106 cells/mL, 0.91 ± 0.02 × 106 cells/mL, and 0.89 ± 0.03 × 106 cells/mL, with specific growth rates of µ = 0.21/day;, µ = 0.20/day; µ = 0.15/day and µ = 0.17/day after 7 days of culture, respectively. In AGP 15% and 20% nutrient media, T. pseudonana microalga reached maximum cell densities of 1.12 ± 0.04 × 106 cells/mL, 1.20 ± 0.05 × 106 cells/mL with specific growth rates of µ = 0.18/day and µ = 0.30/day after 7 and 6 days of culture, respectively. In AGP 5%, 10%, and 25% nutrient media, T. pseudonana culture reached maximum cell densities of 0.75 ± 0.03 × 106 cells/mL, 0.97 ± 0.03 × 106 cells/mL, and 0.98 ± 0.01 × 106 cells/mL with specific growth rates of 0.11/day, 0.20/day, and 0.20/day after 7,8 and 8 days of culture,
respectively. AGP 20% nutrient medium produced the highest biomass and logarithmic cumulative cell concentrations per day and can be used in future experiments.


Keywords


Thalassiosira pseudonana, AGP, conway, microalga, TMRL.



DOI: https://doi.org/10.15625/2615-9023/v40n2se.11595 Display counter: Abstract : 91 views.

 

                 

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