Cultivation of leaf-tissue of Polyscias fruticosa (L.) Harms for quantity of saponin accumulation

Nguyen Trung Hau, Le Thi Nhu Thao, Tran Van Minh


Polyscias fruticosa (L.) Harms is the medicinal plant, popularly cultivated in south-east asia and using as functional-foods. Besides of plantlets micropropagation for area development, hairy-root culture was the techniques manipulated for saponin accumulation. The leaves of young Dinh lang 1-year trees were used as culture materials. Sterilisation with javel water diluted to 50% in 10 minutes gave good results. MS medium supplemented with 1 mg/l NAA was favored for hairy-root induction from leaves tissue cultivation. Treatments with 2.4-D showed that hairy-root was not performed and induced callus strongly. Hairy-root was proliferation strongly in the MS medium supplemented with 0.5 mg/l NAA. Hairy-root cultivated on medium supplemented with IBA was weakly and thin in comparison with NAA. Kinetin was not affected on hairy-root proliferation. Quantity of oleanolic acid and saponin in proliferated hairy-root by HPLC method to show oleanolic acid 40,1 µg/g and saponin 396,2 µg/g accumulation.


Polyscias fruticosa, accumulation, hairy-root, proliferation, saponin

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