Establishment of a procedure to determine the content of 17 amino acids in soft-shell crab (Scylla sp.)

Nguyen Phuong Lan, Do Thi Thanh Trung, Van Thu Vu, Le Tat Thanh

Abstract


Mud crab Scylla sp. is a common sea crab species in Vietnam as well as in Asia Pacific. Today,
mud crabs are raised on a large scale to be harvested at the soft molting stage because of the high economic value of the finished shell crabs. At present, the processing of soft shell crabs is limited to whole packaging and exporting. However, 30% of soft-shelled crabs in processing often lose their feet and claws, which reduce production costs. Therefore, it is necessary to study the technology of processing soft-shell crabs to improve the value of soft-shelled crab products. Recently, the application of enzymes in processing has brought many benefits such as being environmentally friendly and creating many bioactive substances. In this journal, we built the procedure to determine amino acid content in the processing of Scylla sp. to ensure the quality of products obtained after processing. This procedure based on HPLC using a fluorescence reader. The results showed that the amino acid content after hydrolysis process by enzyme technology reached 65.58% dry weight and contains many valuable amino acids such as lysine, leucine, valine, methionine, histidine.

Keywords


Enzyme technology, hydrolysis, mud crab, soft-shell crab

Full Text:

PDF

References


Harun Z, Amin AM, Sarbon NM, Zaino MKM, Sharmin KN (2017) Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum antioxidant activity using response surface methodology. Malays Appl Biol 46(3): 15–21.

Ma X, Zhao D, Li X, Meng L (2015) Chromatographic method for determination of the free amino acid content of chamomile flowers. Pharmaco Magaz 11 (41): 176-179.

Mullally MM, O’Callaghan DM, FitzGerald RJ, Donnelly WJ, Dalton JP (1994) Proteolytic and peptidolytic activities in commercial pancreatic protease preparations and their relationship to some whey protein hydrolysate characteristics. J Agricult Food Chem 42(12): 2973–2981.

Nguyen Thi Phuong Lan, Do Thi Thanh Trung, Van Thu Vu, Dao Thi Dung, Le Tat Thanh (2018) Establishing the hydrolysis procedure of soft-shell crab (Scylla sp.) in Vietnam. Vietnam J Sci Technol 56 (2A): 201-208.

Sarower MG, Bilkis S, Rauf MA, Khanam M, Islam MS (2013) Comparative biochemical composition of natural and fattened mud crab Scylla serrata. J Sci Res 5(3): 545-553.

Vilasoa-Martinez M, López-Hernández J, Lage-Yusty M (2007) Protein and amino acid contents in the crab, Chionoecetes opilio. Food Chem 103: 1330–1336.

Xugan Wu (2010) Comparison of gender differences in biochemical composition and nutritional value of various edible parts of the blue swimmer crab. J Food Comp Anal 23: 154–159.




DOI: https://doi.org/10.15625/1811-4989/18/3/14484 Display counter: Abstract : 67 views. PDF : 31 views.