Evaluation of the sperm separability of blanc-blue-belge bull by swim-up method and in vitro embryo production with hybrid Zebu bovine oocyte in Vietnam

Nguyen Huu Duc, Pham Thu Giang, Tran Thi Binh Nguyen, Bui Dai Phong

Abstract


The objective of this study was to determine the right conditions for the separation of Blanc-Blue-Belge bovine sperm (BBB) by swim-up mothed; determine the maturity of hybrid Zebu bovine eggs; and culture of embryos after in vitro fertilization. After 60-80 minutes of swim-up in CAP-05, BBB bovine sperms were healthy, straight movement and separated with a concentration of 106 sperm/ml. Hybrid Zebu bovine eggs developed and matured in the maturation medium with the basic medium TCM-199 supplemented with 10% calf serum, FSH (0.75 µg / ml), LH (0.15 µg / ml) and Estradiol (2.5 µg / ml), the results showed that the IVM-08 medium had significantly higher maturation rates than IVM-03, the proportion of mature eggs reached 71,11% compared to 51.69%, respectively (P <0.01). In vitro fertilization of hybrid Zebu bovine egg in IVF-08 medium. In vitro fertilized embryos (BBB x hybrid Zebu) developed from bovine sperms separated by the swim-up method achieved a better rate of morula-blastocysts in IVC-09 than IVC-06 medium, 21.68% compared to 8.56%, respectively (P <0.01). The conclusion was that the suitable conditions for BBB bovine sperm separation and in vitro embryo production (BBB x hybrid Zebu) were determined. This is the premise to create bovine semen, BBB bovine embryos with defined gender.

Keywords


BBB bovine sperm, separation, swim-up, hybrid Zebu bovine oocyte, in vitro fertilization, in vitro fertilization, in vitro culture, in vitro embryo production

Full Text:

PDF


DOI: https://doi.org/10.15625/1811-4989/18/1/15265 Display counter: Abstract : 101 views. PDF : 60 views.