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Thu nhận xenlulozơ từ bã mía cho sản xuất etanol sinh học theo phương pháp xử lý với axit axetic

Lê Quang Diễn, Phạm Tuấn Anh, Tô Kim Anh, Nguyễn Thị Minh Nguyệt

Abstract


Sugarcane bagasse contains more than 40 % of cellulose; this material presents a potential feedstock for bioethanol production. In this study, the two-stage treatment of bagasse was used for preparation of cellulosic pulp. Firstly, sugarcane bagasse was treated by mixture of acetic acid and hydrochloric acid (99/1 v/v) in conditions of liquor-tobagasse ratio (10:1) l/kg, temperature (80-90 oC) and 60-90 mins of cooking. Secondary, pretreated bagasse was submerged in sodium hydroxide solution (0.75-1.0 % w/w), at temparature of 80-90 oC, for 30 mins. The yield of cellulosic pulp, which presents 80-85 % purity, was 51-53% of over dry bagasse. Enzymatic saccharification of pretreated bagasse by Novozymes commercial enzyme Cellic®CTec2 with dosage of 45 FPU/g of pretreatred bagasse achieved the reduce sugar yield an average 36.2-38.0 % of over dry bagasse. Fermentation of sugars has been succesful. Study on chemical recovery showed that at laboratory conditions more than 75 % of consumed acetic acid and hydrochloric acid can be recovered by vacuum distillation and can be reused for obtaining of cellulose.


Keywords


Bagasse, acetic acid treatment, enzymatic saccharification, bioethanol, reduce sugars

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