Electrosynthesis of a poly(1,5-diaminonaphthalene) - polypyrrole nanowire bilayer for trichlorfon insecticide biosensing

Nguyen Van Anh, Nguyen Le Huy, Do Phuc Quan, Vuong Thi Bich Lien, Nguyen Tuan Dung, Tran Dai Lam

Abstract


In this study, an acetylcholinesterase enzyme biosensor was developed based on a bilayer of poly(1,5-diaminonaphthalene) and polypyrrole nanowire structures modifying carbon screen-printed electrodes (SPEs). A polypyrrole nanowire inner layer was electrodeposited on the surface of SPEs to enhance conductivity and specific areas. A poly(1,5-diaminonaphthalene) outer layer was used for immobilizing acetylcholinesterase through glutaraldehyde agent. On the basis of the inhibition of organophosphate pesticides on the enzymatic activity of acetylcholinesterase enzyme, the acetylcholinesterase-immobilized bilayer of the conductive polymer electrode was designed for electrochemical determination of trichlorfon insecticide, one of the popular organophosphate pesticides.

Keywords. Acetylcholinesterase, biosensors, poly(1,5-diaminonaphthalene), polypyrrole nanowire, trichlorfon.


Keywords


Acetylcholinesterase, biosensors, poly(1,5-diaminonaphthalene), polypyrrole nanowire, trichlorfon

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