EVALUATION OF ANTIOXIDANT AND ANTI-AGING EFFICACIES OF COFFEA ROBUSTA EXTRACT ON HUMAN FIBROBLAST

Nguyen Thi Ngoc Hoi

Abstract


The extract of Coffea robusta was evaluated via the antioxidant and anti-aging efficacies on human dermal fibroblast. Coffea robusta green beans harvested in Buon Ma Thuot - Vietnam were roasted and extracted by hot water. The anti-oxidant activity of the extract was measured by the DHHP assay. Additionally, the extract was dosed to fibroblast and proteome analysis using 2-D was conducted to observe the changes of proteins involving in aging process. The results show that the extract is a strong anti-oxidant factor with low IC50 value for DPPH scavenging (33.2 mg/L), and significant up-regulation of 5 natural antioxidant enzymes (GPX2, GCLM, PRDX4, GSTA1 and NQO1), as well as down-regulation of 2 supporting-free-radicals proteins (NF-kB1 and TNF-alpha) of human fibroblast.  Furthermore, the extract is a potential anti-aging factor with the down-regulation of 4 proteins related to the degradation of elastin and dermal extracellular matrix (CELA2B, CELA3A, CELA3B and MMP3).


Keywords


anti-aging, anti-oxidant, Coffea robusta, human fibroblast.

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DOI: https://doi.org/10.15625/2525-2518/55/5A/12176 Display counter: Abstract : 224 views. PDF : 138 views.

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Bioteknologi Agrikultur

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Published by Vietnam Academy of Science and Technology