ISOLATION AND SELECTION OF AFLATOXINS PRODUCING ASPERGILLUS FLAVUS FROM PEANUT

Le Thi Phuong Thao

Abstract


Aspergillus flavus is known as the main producer of carcinogenic aflatoxins. The presence of this fungus and aflatoxins is an important impactson food safety, human and animal health. Peanut is one of the most important crops in Vietnam and  is suitable to substrate for aflatoxins producing fungus growth. The isolation and identification of Aspergillus flavus species from peanut is an initial step for further study in Aflatoxins contamination control in peanut. 28 peanut samples selected in Luc Nam district - Bac Giang province were used for fungus isolation. The isolated fungus were purified, conducted DNA extract, amplified PCR products then performed sequencing ITS gene and used BLAST software to check alignment to identification the strains. As the result, 4 strains alignedwith Aspergillus flavus strains from National Center for Biology Information - United Nation such as: TUHT115, KP214054.1, MTCC 8654, ZJ4-A. Those strains were checked and confirmed the aflatoxins production in peanut samples.


Keywords


Aspergillus flavus, Aflatoxins, peanut, Aflatoxin B1, Aflatoxin B2.

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DOI: https://doi.org/10.15625/2525-2518/55/5A/12187

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Bioteknologi Agrikultur

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Published by Vietnam Academy of Science and Technology