Rhodosporidium sp. GROWTH IN MOLASSES MEDIUM AND EXTRACTION OF ITS ASTAXANTHIN BY USING HCl

Tran Quang Vinh

Abstract


Astaxanthin is classified as a xanthophyll-carotenoid, which is red-orange colour and powerful antioxidant activity. In this study, astaxanthin was collected from Rhodosporidum sp. by pilot culture (10 liters). Molasses medium was investigated with urea ((NH4)2CO), magnesium sulfate heptahydrate (MgSO4.7H2O) and potassium dihydrogen phosphate (KH2PO4) at different concentrations. Astaxanthin was extracted by using chlorhydric acid (HCl) method.

The highest dried yeast biomass was 8.3682 g/l culture supernatant and astaxathin was 1.932 g/l culture supernatant by molasses medium containing 20 g/l sugar, 0.5 g/l ((NH4)2CO, 3 g/l MgSO4.7H2O and 10 % (v/v) inoculum. HCl extraction method was mixed 10 mg biomass: 1 ml HCl 0.6 N and incubated at 70 oC, 150 minutes.


Keywords


astaxanthin, Rhodosporidium sp., pilot, molasses medium, HCl extract.

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DOI: https://doi.org/10.15625/2525-2518/55/1A/12377 Display counter: Abstract : 225 views. PDF : 180 views.

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Bioteknologi Agrikultur

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Published by Vietnam Academy of Science and Technology