IN VITRO EVALUATION OF ANTIBACTERIAL ACTIVITY OF GARLIC ALLIUM SATIVUM AGAINST POULTRY PATHOGENS AND EFFECT OF GARLIC SUPPLEMENTATION ON DUCKLING GROWTH PERFORMANCE

Tien Cuong Nguyen, Thi Anh Phuong Chu, Hai Van Nguyen

Abstract


ABSTRACT-QMFS2019

Poultry production provides source of protein and contributes an important income for Vietnamese farmers. Among the poultry in Vietnam, ducks account for 27.3% of head of poultry and even 55.7% in Mekong Delta region. Along with the development of rearing ducks, bacterial, viral and fungal diseases occurring in the two last decades induced bad effect for poultry producer. Escherichia coli, Salmonella enterica, Streptococcal or Pasteurella act as major pathogenic bacteria in duck. The aims of this study were to investigate the antibacterial activity of garlic Allium sativum against E. coli, Staphylococcus aureus, Salmonella Typhimurium and to evaluate the effect of garlic on growth performance of duck from 1-28 old-days. The results indicated that fresh garlic and dried garlic powder showed inhibitory effect against pathogenic tested strains from 2% and 4% w/v, respectively. The inhibition zones and the minimal inhibitory concentration (MIC) values of garlic extract ranged from 11.3-28.3 mm and 0.02-0.2 g/ml, respectively. After 28 days of diet with garlic supplemented, D3 (2% of fresh garlic in water) showed significantly different in weight gain (WG), feed conversion ratio (FCR), protein efficiency ratio (PER), average daily weight (ADW); whereas, D2 (2% of garlic powder in basal diet) only possessed a difference significant in feed consumption (FC) compared to the D1 (control without garlic supplementation). The obtained results demonstrated the potential of garlic application in poultry production.


Keywords


Garlic, duck, antibacterial activity, growth performance, poultry production

Full Text:

PDF

References


Men B.X. - Duck farming systems and avian influenza in the Mekong delta of Vietnam, FAO Smallholder Poultry Production Paper, 1 (2010) 1-8.

Nhung N.T., Chansiripornchai N., Carrique-Mas J.J. - Antimicrobial resistance in bacterial poultry pathogens: a review, Frontiers in Veterinary Science, 4 (2017) 126.

Kuldeep D., Shyma K.L., Saminathan M., Hari A.S., Karthik K., Ruchi T., Rifat U.K., Mahmoud A., Mayada R.F., Gazi M.A., Vito L., Vincenzo T. - Multiple beneficial applications and modes of action of herbs in poultry health and production-A review, International Journal of Pharmacology, 11 (2015) 152-176.

Puvača N., Ljubojević D., Kostadinović L., Lukač D., Lević J., Sanja T. P., Djuragic O. - Spices and herbs in broilers nutrition: Effects of garlic (Allium sativum L.) on broiler chicken production, World's Poultry Science Journal, 71 (2015) 533-538.

Mansoub N.H. - Comparative effects of using garlic as probiotic on performance and serum composition of broiler chickens, Annals of Biological Research, 2(3) (2011) 486-490.

Balouiri M., Sadiki M., Ibnsouda S.K. - Methods for in vitro evaluating antimicrobial activity: A review, Journal of Pharmaceutical Analysis, 6(2) (2016) 71-79.

Durairaj S., Srinivasan S., Lakshmanaperumalsamy P. - In vitro antibacterial activity and stability of garlic extract at different pH and temperature, Electronic Journal of Biology, 5(1) (2009) 5-10.

Khashan A.A. - Antibacterial activity of garlic extract (Allium sativum) against Staphylococcus aureus in vitro, Global Journal of Bio-Science and Biotechnology, 3(4) (2014) 346-348.

Gull I., Saeed M., Shaukat H., Aslam S.M., Samra Z.Q., Athar A.M - Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria, Annals of Clinical Microbiology and Antimicrobials, 11(1) (2012) 8.

Sivam G.P., Lampe J.W., Ulness B., Swanzy S.R., Potter J.D. - Helicobacter pylori - in vitro susceptibility to garlic (Allium sativum) extract, Nutrition and Cancer, (1997) 118-121.

Booyens J., Labuschagne M.C., Thantsha M.S. - In vitro antibacterial mechanism of action of crude garlic (Allium sativum) clove extract on selected probiotic Bifidobacterium species as revealed by SEM, TEM, and SDS-PAGE analysis, Probiotics and Antimicrobial Proteins, 6(2) (2014) 82-87.

Eja M.E, Asikong B.E., Abriba C., Arikpo G.E., Anwan E.E., Enyi-Idoh K.H. - A comparative assessment of the antimicrobial effects of garlic (Allium sativum) and antibiotics on diarrheagenic organisms, Southeast Asian Journal of Tropical Medicine and Public Health, 38(2) (2007) 343-348.

Elagib H.A.A., El-Amin W.I.A., Elamin K.M., Malik H.E.E. - Effect of dietary garlic (Allium sativum) supplementation as feed additive on broiler performance and blood profile, Journal of Animal Science Advances, 3(2) (2013) 58-64.

Fadlalla I.M.T., Mohammed B.H., Bakhiet A.O. - Effect of feeding garlic on the performance and immunity of broilers, Asian Journal of Poultry Science, 4(2010) 182-189.




DOI: https://doi.org/10.15625/2525-2518/57/3B/14045 Display counter: Abstract : 647 views. PDF : 219 views.

Refbacks

  • There are currently no refbacks.


Bioteknologi Agrikultur

Index: Google Scholar; Crossref; VCGate; Asean Citation Index

Published by Vietnam Academy of Science and Technology