OPTIMIZATION OF CONDITIONS FOR CAROTENOIDS EXTRACTION FROM SHRIMP WASTE USING ORGANIC SOLVENT

Le Thanh Ha, Le Duy Thang

Abstract


In this study, factors affecting the extraction yield of carotenoids from dry shrimp waste by organic solvents such as ratio of hexane / acetone, ratio of solvent / shrimp waste, extraction temperature, extraction time, extraction method such as dynamic or static have been studied. The results showed that the solvent ratio hexane: acetone = 3: 1 gave the highest carotenoid yield. In this ratio of solvent’s mixture, the yield reached highest at temperature 60 °C for 2 hours, which was 44,64 µg / g raw shrimp waste (d.b.) (ratio of solvent to raw material 3/1). Ultrasound or vortexing gave higher extraction yield than in static conditions, which was 1.5- to 1.8- fold increase, respectively. At the ratio of solvent: dried shrimp = 4: 1, the amount of carotenoid recovered at 60°C for 2 hours reached 57.4 µg / g. However, if the shrimp waste was hydrolyzed with Alcalase at 50°C for 4 hours before extraction by solvent, the amount of carotenoid recovered achieved 149 µg / g of raw material

Keywords


Alcalse, Carotenoid, Penaeus vannamei, shrimp waste, extraction.

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References


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DOI: https://doi.org/10.15625/2525-2518/57/3B/14111 Display counter: Abstract : 273 views. PDF : 107 views.

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