Purification and bioactivity of fucoidan from kappaphycus alvarezii alga

Nhon Thi Ngoc Hoang, Anh Thi Hong Le, Kiet Tuan Nguyen, Chi Thi Minh Nguyen, Ngoc Thi My Le, Tien Pham Cam Nguyen

Abstract


Kappaphycus alvarezii is a type of seaweed containing numerous carbohydrate with biological activities, especially fucoidan. About chemical aspect, fucoidan is fucose polysaccharides (FCSPs) with diverse biological activities including antioxidant, anticoagulant, anti-inflammatory, antiviral, antitumor... Fucoidan is found not only in brown algae but also in K. alvarezii alga from the central coast of Vietnam. In this study, fucoidan purification, fucoidan structure and biological activities of K. alvarezii alga (collected at Dam Mon area, Khanh Hoa province) were investigated. The yield recovery of two-stage precipitation by ethanol 99% was 84.4%. The protocol of fucoidan purification by ion-exchange chromatography was applied with tris-HCl buffer, elution solvent NaCl 1M; The yield was 60.99%. The structure of fucoidan from K. alvarezii was determined by 1H-NMN spectrum with the specific characteristic properties of fucoidan. The antioxidant activity of the purified fucoidan via IC50 was 303.51±0.12 μg/mL (DPPH assay), 299.97±3.12 μg/mL (ABTS essay). The purified fucoidan was shown better ability in cases of anti-coagulant, antibacterial activities than that of the crude extract. The anti-coagulation time of the purified fucoidan was 22.52 minutes. The minimum inhibitory concentrations (MICs) of the antibacterial activity (Bacillus cereus, Escherichia coli) were 400μg/mL. MICs was 500 μg/mL (Aspergillus flavus) and 600 μg/mL (Aspergillus niger).


Keywords


bioactivity, fucoidan, ion-exchange chromatography, K. alvarezii, purification.

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Li B., Lu F., Wei X., Zhao R. - Fucoidan: structure and bioactivity, Mol. 13 (2008) 1671-1695.

Marxen K., Vanselow K.H., Lippemeier S., Hintze R., Ruser A., and Hansen U.P. - Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis of spectrophotometric measurements, Sensors, 7 (2007) 2080-2095.

BlondinC. and Agostiniz A. - Biological activities of polysaccharides from marine algae, Drugs Fut, 20 (1995) 1237-1249.

Rupérez P., Ahrazem O. and Leal J.A. - Potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed F. vesiculosus, J. Agric. Food Chem., 50 (2002) 840-845.

Wang J., Zhang Q., Zhang Z., and Li Z. - Antioxidant activity of sulfated polysaccharide fractions extracted from L. japonica, Int J Biol Macromol, 42 (2008) 127-132.

Chotigeat W., Tongsupa S., Supamataya K., and Phongdara A. - Effect of fucoidan on disease resistance of black tiger shrimp, Aqu., 233 (2004) 23-30.

Avendaño H. R., Lody M., and Riquelme C.E. - Producción de substancias inhibitorias entre bacterias de biopelículas en substratos marinos, Rev Biol Mar Oceanog, 40 (2005) 117-125.

Dinh N. H. - Sargassum algae at Hon Chong beach-Nha Trang province, Science and Technics Publishing House, 1991.

Marudhupandi T., Kumar T.A., Senthil S.L., and Devi K.N. - In vitro antioxidant properties of fucoidan fractions from S. tenerrimum, Pak J Biol Sci, 17 (3) (2014) 402.

Dische Z. and Shettles L.B. - A specific color reaction of methyl pentoses and a spectrophotometric micro method for their determination, J Biol Chem., 175 (2) (1948) 595-603.

Bilan M.I., Grachev A.A., Ustuzhanina N.E., Shashkov A.S., Nifantiev N.E., Usov A.I - Structure of a fucoidan from the brown seaweed F. evanescens, Carbohy. Res., 337 (8) (2002)719-30.

Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., and Rice-Evans C. - Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic Biol Med, 26 (1999) 1231-1237.

Kamble S.P., Gaikar R.B., Padalia R.B., and Shinde K.D. - Extraction and purification of C-phycocyanin from dry Spirulina powder and evaluating its antioxidant, anticoagulation and prevention of DNA damage activity, J. Appl. Pharm. Sci. 3 (2013) 149.

Mak W., Hamid N., Liu T., Lu J., and White W. - Fucoidan from New Zealand U. pinnatifida: Monthly variations and determination of antioxidant activities, Carb. Polym., 95 (1) (2013) 606-614

Isnansetyo A., Lutfia F.N.L., Nursid M., and Susidarti R.A. - Cytotoxicity of fucoidan from three tropical brown algae against breast and colon cancer cell lines, Phar Jl, 9 (1) (2017).

Kim W.J, Kim S.M, Kim H.G., Oh H.R., Lee K.B., Lee Y.K, et al. - Purification and anticoagulant activity of a fucoidan from Korean U. pinnatifida sporophyll, Algae, 22 (3) (2007) 247-252.

Berteau O. and Mulloy B. - Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide, Glycobiology, 13 (6) (2003) 29-40.

Chevolot L., Foucault A., Chaubet F., Kervarec N, Sinquin C, Fisher AM, Boisson-Vidal C. - Further data on the structure of brown seaweed fucans: relationships with anticoagulant activity, Carbohy. Res., 319 (1-4) (1999) 154-165.

Ponce A.G. Roursa S.I, Valle C.E, and Moreira M.R. – Antimicrobial and antioxidant activities of edible coatings enriched with natural plant extracts, Postharvest Biol Tec, 49 (2008) 294-300.

Grierson D. and Afolayan A. – Antibacterial activity of some indigenous plants used for the treatment of wounds in the Eastern Cape, South Africa, J Ethnopharmacol 66 (1999) 103-106.

Berteau O. and Mulloy B. - Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide, Glycobiology, 13 (2013) 29-40.

Eluvakkal T., Shanthi N., Murugan M., and Arunkumar K. - Extraction of antibacterial substances, galactofucoidan, and alginate successively from the Gulf of Mannar brown seaweed S. wightii, Indian j. nat. prod. resour. 5 (3) (2014) 249-257.

Pereira M.S., Melo F.R., and Mourão P.A. - Is there a correlation between structure and anticoagulant action of sulfated galactans and sulfated fucans, Glycobiology, 12 (2002) 573-580.




DOI: https://doi.org/10.15625/2525-2518/57/3B/14219 Display counter: Abstract : 429 views. PDF : 172 views.

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