Optimization of Extraction of Total Phenolic and Total Flavonoid from Seeds of Alpinia blepharocalyx K. Schum. Using The Response Surface Methodology

Thanh Tan Nguyen, Huyen Thi Thanh Mai, Tuan Ngoc Nguyen, Quang Dinh Tran, Thang Dinh Tran


Seeds of Alpinia blepharocalyx contain high amounts of phenolic and flavonoid compounds with potential antioxidant properties. In this study, the effects of the extraction method on the recovery of phenolic and flavonoid compounds from Alpinia blepharocalyx seeds were investigated. Response surface methodology (RSM) has been used to optimize the extraction conditions of total phenolic and total flavonoid from seeds of Alpinia blepharocalyx. A Box-Behnken design was used to investigate the effects of four independent variables, namely extraction temperature (°C), extraction time (min), (v/m) and ethanol concentration (%) on the responses: total phenolic content (TPC) and yield. The optimal conditions obtained from response RSM were 52.66 % v/v for the solvent composition, 62.34 (°C) for extraction temperature, 34.48/1 (ml/g) for solvent/material ratio and 125.42 (min) for extraction time. The experimental values of TPC, TFC and yield were 39.31±0.05 mgGAE/g, 12.75±0.07 mgCE/g and 6.97±0.05%, respectively.   


Alpinia blepharocalyx, total phenolic content (TPC), total flavonoid content (TFC), response surface methodology (RSM), extraction.

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DOI: https://doi.org/10.15625/2525-2518/57/3B/14443 Display counter: Abstract : 594 views. PDF : 160 views.


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