Thi Hanh Nguyen Vu, Quang Huy Nguyen, Thi Thu Hang Le, Son Chu-Ky, Quyet Tien Phi


This study determined the optimal nutrient and environmental conditions for the production of antimicrobial and cytotoxic agents by Streptomyces cavourensis YBQ59 during the fermentation process. The antimicrobial and cytotoxic activities of eluted fractions based gradient solvents via silica gel column of chromatograph were also evaluated. S. cavourensis YBQ59 exhibited strong, board-spectrum antimicrobial activities under fermentation conditions as follows: MT6 medium with soluble starch and soybean powder as carbon and nitrogen sources, respectively, temperature 30oC, initial pH 7.0, 20% DO concentration and with 5% initial seed culture. The kinetic of fermentation showed that the antimicrobial and cytotoxic activities were highest at between 72h and 78h. The fraction 25/1 D-M (dichloromethane –methanol) exhibited the highest antimicrobial effect against methicillin-resistant Staphylococcus aureus ATCC 33591 and methicillin-resistant Staphylococcus epidermidis ATCC 35984 with minimum inhibitory concentrations of 9.4 µg/ml and 6.4 µg/ml, respectively. The fraction 10/1 D-M had strong cytotoxic effects towards multidrug-resistant A459 and H1299 lung carcinoma cell lines with the cell viability of 11.3% and 12.4%, respectively. In conclusion, S. cavourensis YBQ59 would be a potential producer of valuable bioactive compounds that may have a board application in pharmaceutical industry and agriculture (biocontrol, livestock, food safety and quality management)


antimicrobials, Cinnamomum cassia, endophytic actinomycete, fermentation, food safety, Streptomyces cavourensis

Full Text:



Ventola C. L. - The antibiotic resistance crisis: part 1: causes and threats. Pharm Therapeut. 40 (4) (2015) 277-283.

Zaman S. B., Hussain M. A., Nye R., Mehta V., Mamun K. T., and Hossain N. - A Review on Antibiotic Resistance: Alarm Bells are Ringing. Cureus. 9 (6) (2017) e1403.

Vu T., Nguyen Q. H., Dinh T. M. L., Quach N. T., Khieu T. N., Hoang H., Son C.-K., Vu T. T., Chu H. H., and Lee J. - Endophytic actinomycetes associated with Cinnamomum cassia Presl in Hoa Binh province, Vietnam: Distribution, antimicrobial activity and, genetic features. J Gen Appl Microbiol. (2019).

Vu H., Nguyen D. T., Nguyen H. Q., Chu H. H., Chu S. K., Van Chau M., and Phi Q.-T. - Antimicrobial and cytotoxic properties of bioactive metabolites produced by Streptomyces cavourensis YBQ59 isolated from Cinnamomum cassia Prels in Yen Bai Province of Vietnam. Curr Microbiol. 75 (10) (2018) 1247-1255.

Song Q., Huang Y., and Yang H. - Optimization of fermentation conditions for antibiotic production by Actinomycetes YJ1 strain against Sclerotinia sclerotiorum. J Agr Sci 4(7) (2012) 95-102.

Qin S., Li J., Chen H.-H., Zhao G.-Z., Zhu W.-Y., Jiang C.-L., Xu L.-H., and Li W.-J. - Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl Environ Microbiol. 75 (19) (2009) 6176-6186.

Janso J. E., and Carter G. T. - Biosynthetic potential of phylogenetically unique endophytic actinomycetes from tropical plants. Appl Environ Microb. 76 (13) (2010) 4377-4386.

Conti R., Cunha I. G., Siqueira V. M., Souza-Motta C. M., Amorim E. L., and Araujo J. M. - Endophytic microorganisms from leaves of Spermacoce verticillata (L.): Diversity and antimicrobial activity. J Appl Pharm 2(12) (2012) 17-22.

Wang X., Huang L., Kang Z., Buchenauer H., and Gao X. - Optimization of the fermentation process of Actinomycete strain Hhs. 015 T. BioMed Res Int. 2010 (0) (2010) 1-10.

Khan J. A., and Patel A. S. - Extraction and purification of antibacterial metabolites from actinomycetes spp. isolated from soil sample. Int J Pharmaceut. 3 (10) (2011) 63-71.

Shenpagam H. N., Devi D. K., Sinduja G., and Sandhya R. - Isolation of endophytic actinomycetes from medicinal plants and its mutational effect in biocontrol activity. International Journal of Pharmaceutical Sciences and Research. 3 (11) (2012) 4338.

Holder I., and Boyce S. - Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. Burns. 20 (5) (1994) 426-429.

Khieu T.-N., Liu M.-J., Nimaichand S., Quach N.-T., Chu-Ky S., Phi Q.-T., Vu T.-T., Nguyen T.-D., Xiong Z., and Prabhu D. M. - Characterization and evaluation of antimicrobial and cytotoxic effects of Streptomyces sp. HUST012 isolated from medicinal plant Dracaena cochinchinensis Lour. Front Microbiol. 6 (2015) 574.

El-Naggar M. Y., Hassan M. A., Said W. Y., and Samy A E.-A. - Effect of support materials on antibiotic MSW2000 production by immobilized Streptomyces violatus. J Gen Appl Microbiol. 49 (4) (2003) 235-243.

Wu J. Y., Huang J. W., Shih H. D., Lin W. C., and Liu Y. C. - Optimization of cultivation conditions for fungichromin production from Streptomyces padanus PMS-702. J Chin Inst Chem Engi 39 (1) (2008) 67-73.

Reddy K., Nurdijati S., and Salleh B. - An overview of plant-derived products on control of mycotoxigenic fungi and mycotoxins. Asian J Plant Sci. 9 (3) (2010) 126-133.

Hassan M. A., El-Naggar M. Y., and Said W. Y. - Physiological factors affecting the production of an antimicrobial substance by Streptomyces violatus in batch cultures. Egypt J Biology. 3 (1) (2001) 1-10.

Oskay M. - Effects of some environmental conditions on bomass and atimicrobial metabolite production by Streptomyces Sp., KGG32. Inter J Agr Biol 13 (3) (2011) 317-324.

Yu J., Liu Q., Liu Q., Liu X., Sun Q., Yan J., Qi X., and Fan S. - Effect of liquid culture requirements on antifungal antibiotic production by Streptomyces rimosus MY02. Bioresource Technol. 99 (6) (2008) 2087-2091.

Dezfully N. K., and Ramanayaka J. G. - Isolation, identification and evaluation of antimicrobial activity of Streptomyces flavogriseus, strain ACTK2 from soil sample of Kodagu, Karnataka State (India). Jundishapur J Microb. 8 (2) (2015) 1-8.

Abdelghani T. - Production of antibacterial and antifungal metabolites by (S. albovinaceus) strain no. 10/2 and media optimization. Am Int J Biol. 5 (1) (2017) 1-24.

Nalini M., and Prakash H. - Diversity and bioprospecting of actinomycete endophytes from the medicinal plants. Lett Appl Microbiol 64 (4) (2017) 261-270.

Machavariani N., Ivankova T., Sineva O., and Terekhova L. - Isolation of endophytic actinomycetes from medicinal plants of the Moscow region, Russia. World Appl Sci J. 30 (11) (2014) 1599-1604.

Strobel G., and Daisy B. - Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol R. 67 (4) (2003) 491-502.

Ezra D., Castillo U. F., Strobel G. A., Hess W. M., Porter H., Jensen J. B., Condron M. A., Teplow D. B., Sears J., and Maranta M. - Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp.(MSU-2110) endophytic on Monstera sp. Microbiology. 150 (4) (2004) 785-793.

Igarashi Y., Trujillo M. E., Martínez-Molina E., Yanase S., Miyanaga S., Obata T., Sakurai H., Saiki I., Fujita T., and Furumai T. - Antitumor anthraquinones from an endophytic actinomycete Micromonospora lupini sp. nov. Bioorg Med Chem Lett 17 (13) (2007) 3702-3705.

DOI: https://doi.org/10.15625/2525-2518/57/3B/14501 Display counter: Abstract : 375 views. PDF : 230 views.


  • There are currently no refbacks.

Bioteknologi Agrikultur

Index: Google Scholar; Crossref; VCGate; Asean Citation Index

Published by Vietnam Academy of Science and Technology