Isolation and screening of endophytic bacteria from Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv) for biosynthesis β-glucosidase

Authors

  • Nguyen Thi Tam Viện Công Nghệ Sinh Học - Viện Hàn Lâm Khoa Học và Công Nghệ Việt Nam
  • Nguyen Phu Tam
  • Vu Thi Hanh Nguyen
  • Nguyen Khac Hung
  • Chu Nhat Huy
  • Pham Bich Ngoc
  • Chu Hoang Ha
  • Phi Quyet Tien

DOI:

https://doi.org/10.15625/0866-7160/v40n2.10862

Keywords:

Biotransformation, Endophytic bacteria, Ginsenoside Rb1, Ngoc Linh ginseng, Panax vietnamensis.

Abstract

Biotransformation of secondary metabolites from medicinal plants by bacteria has been studied extensively in the recent years. One of the potential sources for screening bacteria is plant endophytic bacteria. In this study, we isolated and screened endophytic bacteria for transformation of ginsenoside Rb1 of Ngoc Linh ginseng collected at village number 2, Tra Linh commune, Nam Tra My district, Quang Nam province. There are 45 strains isolated form rhizobium (24 strains), petioles (8 strains) and leaves (13 strains). After screening, there are 27 strains positive with β-glucosidase test, an enzyme which catalyses the hydrolysis of terminal non-reducing residues in β-glucosides-aglycone linkage of ginsenoside Rb. By evaluating the β-glucosidase activity and identification via 16S rRNA sequence, we choosed four high β-glucosidase acitivity strains SVK32 (Enterobacter sp.); SVK34 (Serratia sp.); SVK37 (Ochrobactrum sp.) and SVK44 (Arthrobacter sp.) for futher study on biotransformating of ginsenoside Rb1 into ginsenoside Rd and Rg3.

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Author Biography

Nguyen Thi Tam, Viện Công Nghệ Sinh Học - Viện Hàn Lâm Khoa Học và Công Nghệ Việt Nam

Phòng Công nghệ Tế bào Thực vật

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Published

06-11-2018

How to Cite

Tam, N. T., Tam, N. P., Nguyen, V. T. H., Hung, N. K., Huy, C. N., Ngoc, P. B., Ha, C. H., & Tien, P. Q. (2018). Isolation and screening of endophytic bacteria from Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv) for biosynthesis β-glucosidase. Academia Journal of Biology, 40(2), 153–161. https://doi.org/10.15625/0866-7160/v40n2.10862

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