Assessment of biocompatibility of acellular vascular grart from porcine carotid artery <i> in vitro </i> and <i> in vivo </i>
Keywords:Acellular vascular graft, carotid artery, decellularization, biocompatibility, Dacron grafts.
Acellular vascular graft (MMVB) is one of the major research trends in cardiovascular tissue engineering because of its high abundance, low-cost and reduced immunogenicity. Biocompatibility is a key factor for its success. In this study, porcine carotids were decellularized by SDS 0,5%, distilled water to produce MMVBs. In vitro biocompatibility was assessed based on ISO 10993-5 (with fibroblasts) and mesenchymal stem cell (TBGTM) growth on lumen after 13 days. MMVBs was then transplanted under rodent skin to evaluate in vivo biocompatibility for a period of 4 weeks. Results showed that a combination of SDS 0.5% for 24 hours and distilled water for 24 hours removed all cells and preserved the extracellular matrix. MMVBs were non-toxic (level 0, according to ISO 10993-5) and supported the growth of TBGTM (peak at day 7). Haematoxylin/Eosin staining showed that inflammation was absent in the 4th week (similar to commercial Dacron grafts). In conclusion, MMVBs have biocompatibility in vitro and in vivo.