A comparison of the cytotoxic activity of extracts from artificial fruiting bodies and mycelial biomass of fungus <i> Cordyceps takaomontana </i> (DL0038A)
Keywords:Biomass, carcinoma, Cordyceps takaomontana, fruit body, fungus.
Cordyceps takaomontana is an insect-parasitic fungus, naturally distributed in the Langbiang mountain, Tam Dao and Ba Vi national parks of Vietnam. This study assessed and compared the in vitro cytotoxic activity of crude extracts from fruit bodies and biomass of artificial C. takaomontana (DL0038A) fungus against human Jurkat T (acute T cell leukemia) and breast carcinoma (MCF-7) cells using the sulforhodamine B (SRB) assay. We obtained the extracts via the ethanol extraction (EtOH extract) and liquid-liquid extraction with four different solvents; petroleum ether (60 – 80oC) (PE), ethyl acetate (EA), butanol (BuOH) and water (W), successively. The result shows the cytotoxic potential of biomass extracts was lower than those of fruit-body extracts. All of the biomass extracts did not determine the IC50 value at 0 – 100 µg/mL concentrations. By contrast, the EA-fruit-body extract displayed the highest cytotoxic activity on MCF-7 and Jurkat T cell lines with IC50 values of 33.88 ± 1.49 µg/mL and 42.10 ± 2.98 µg/mL, respectively. Similarly, the IC50 value of BuOH-fruit-body extract for MCF-7 cells was 85.91 ± 2.28 µg/mL. Interestingly, we need to study further about the cytotoxic mechanisms of ET and BuOH-fruit-body extracts on MCF-7 and Jurkat T cells to possibly use as a promising anticancer agent.