Isolation and characterization of a c-repeat binding factor gene from <i> Tevang-1</i> maize cultivar

Authors

  • Nguyen Thuy Linh Institute of Genome Research, Vietnam Academy of Science and Technology
  • Luu Han Ly Institute of Genome Research, Vietnam Academy of Science and Technology
  • Nguyen Thuy Duong 1. Institute of Genome Research, Vietnam Academy of Science and Technology 2. Graduate University of Science and Technology, Vietnam Academy of Science and Technology
  • Huynh Thi Thu Hue 1. Institute of Genome Research, Vietnam Academy of Science and Technology 2. Graduate University of Science and Technology, Vietnam Academy of Science and Technology

DOI:

https://doi.org/10.15625/0866-7160/v41n3.13782

Keywords:

CBF/DREB1, transcription factor, Tevang-1, maize, cold tolerance.

Abstract

C-repeat binding factor (CBF) proteins are transcription factors involved in plant response to abiotic stresses, especially low-temperature condition. In this research, a CBF3-coding gene was isolated from a cold-acclimation maize variety, Zea mays var. Tevang-1 and denoted as ZmCBF3tv. The isolated gene shared 96.49% homology with the B73-reference gene and had no intron in the coding sequence. By using bioinformatic tools, a number of variations in the nucleotide and amino acid sequences were identified. An alignment between ZmCBF3tv and other CBF/DREB1 proteins from various species revealed functional regions and typical features, such as nuclear localization signal (NLS), the AP2 DNA-binding domain, and acidic-amino-acid-rich segments. Additionally, a phylogenetic analysis based on the AP2 domain showed that the maize CBF3 transcription factor had the highest similarity with that from rice and closely related to other DREB1/CBF protein of monocots. The function of the ZmCBF3tv product is suggested to be a CBF/DREB1 transcription factor.

 

 

 

 

 

Downloads

Download data is not yet available.

Downloads

Published

26-07-2019

How to Cite

Linh, N. T., Ly, L. H., Duong, N. T., & Hue, H. T. T. (2019). Isolation and characterization of a c-repeat binding factor gene from <i> Tevang-1</i> maize cultivar. Academia Journal of Biology, 41(3). https://doi.org/10.15625/0866-7160/v41n3.13782

Issue

Section

Articles

Most read articles by the same author(s)