Cytotoxicity and antioxidant activity of plant extracts from Vietnam
Keywords:Antioxidant, cell cycle arrest, cytotoxycity, plant extracts, reactive oxygen species.
Traditional medicine plays an important role in treatment of human diseases. Extracts from medicinal plants exhibit many valuable biological activities. However, the exploitation and application of the extracts requires knowlege on the mechanism of action. In this study, cytotoxic and antioxidant activites of the methanol and hexane extracts of 09 Vietnamese plants have been studied in vitro on keratinocyte HaCaT cell line. The results showed that all plant extracts had a cytotoxyc effect on HaCaT cells. The hexane extracts showed more potent than the methanol extract. Garcinia mangostana exhibited the best cytotoxicity with IC50 values of 14.42 mg/ml (for hexane extract) và 14.27 mg/ml (for MeOH extract). All tested extracts resulted in the generation of intracellular reactive oxygen radicals in HaCaT cells. Mangifera indica, Cleistocalyx operculatus and Terminalia catappa had the best DPPH radical scavenging activity with EC50 values of 23.0 mg/ml, 27.4 mg/ml and 23.73 mg/ml, respectively. Besides, G. mangostana and T.catappa exhibited capacity of eliminating active oxygen radicals (iROS). The test extracts significantly reduced the number of cells in phase G1 and increased the number of cells in S and G2/M phases. The data obtained in this study are the preliminary results for further studies on the mechanisms of action and therapeutic applications of these plants.
Abdullah A. H., Mohammed A. S., A. R., Mirghani M. E. S., Al-Qubaisi M., 2014. Cytotoxyc effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231cell lines) and bioactive constituents in the crude extract. BMC Compl. Altern. Med, 14: 199.
Abrahim N. N., Kanthimathi M. S., Abdul-Aziz A., 2012. Piper betle shows antioxydant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxyde dismutase. BMC Compl. Altern. Med, 12: 220.
Behera D. R., Bhatnagar S., Mahapatra A. K., 2013. Cytotoxyc and radical scavenging potential of Indian almond (Terminalia catappa) leaf extracts. British Biomed. Bull, 1: 31–39.
Di Paola R. S., 2002. To arrest or not to G2-M cell cycle arrest. Clin. Cancer Res., 8: 3311–3314.
Dosoky N. S., Pokharel S. K., Setzer W. N., 2015. Leaf essential oil composition, antimicrobial and cytotoxyc activities of Cleistocalyx operculatus from Hetauda, Nepal. American J. Essent. Oil Nat.Prod., 3(1): 34–37.
Do Tat Loi, 1991. Medicinal plants and herbs of Vietnam, Medical publishing house.
Huang Y. H., Wu P. Y., Wen K. C., Lin C. Y., Chiang H. M., 2018. Protective effects and mechanisms of Terminalia catappa L. methenolic extract on hydrogen-peroxyde-induced oxydative stress in human skin fibroblasts. BMC Complement. Altern. Med, 18(1): 266.
Li C. Y., Tsai W. J., Damu A. G., Lee E. J., Wu T. S., Dung N. X., 2007. Isolation and identification of antiplatelet aggregatory principles from the leaves of Piper lolot. J. Agr. food Chem., 55(23): 9436–9442.
Mahabusarakam W., Chairerk P., Taylor W. C., 2005. Xanthones from Garcinia cowa Roxb. latex. Phytochemistry, 66(10): 1148–1153.
Moongkarndi P., Kosem N., Kaslungka S., Luanratana O., Pongpan N., Neungton N., 2004, Antiproliferation, antioxydation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line. J. Ethnopharmacol, 90(1): 161‒166.
Matsumoto K., Akao Y., Ohguchi K., Ito T., Tanaka T., Iinuma M., Nozawa Y., 2005. Xanthones induce cell-cycle arrest and apoptosis in human colon cancer DLD-1 cells. Bioorg. Med. Chem., 13(21): 6064–6069.
Padmapriya N., Poonguzhali T. V., 2015. Antioxydant potential from the acetone extractstem and leaves of Piper betle. IJAR, 19–22.
Saraiva dos R., 2012. An in vitro analysis of the total phenolic content, antioxydant power, physical, physicochemical, and chemical composition of Terminalia catappa Linn fruits. Sci. Technol. Aliment, 32(1): 209–213.
Riss T. L., Moravec R. A., Niles A. L., Benink H. A., Worzella T. J., Minor L., 2004. Cell viability assays. In Assay Guidance Manual. Bethesda (MD).
Tailor C. S., Anju G., 2014. Antioxydant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. leaves. American J. Ethnol: 244–249.
Tandon V., Gupta B. M., 2005. Free radicals/reactive oxygen species. JKPractitioner: 143–148.
Tsai S. Y., Chung P. C., Owaga E. E., Tsai I. J., Wang P. Y., Tsai J. I., Yeh T. S., and Hsieh R. H., 2016. Alpha-mangostin from mangosteen (Garcinia mangostana Linn.) pericarp extract reduces high fat-diet induced hepatic steatosis in rats by regulating mitochondria function and apoptosis. Nutr. Metab. (Lond), 13: 88.
Wang H., Joseph J. A., 1999. Quantifying cellular oxydative stress by dichlorofluorescein assay using microplate reader. Free Rad. Boil. Med., 27(5-6): 612–616.
Wu P., Ma G., Li N., Deng Q., Yin Y., Huang R., 2015. Investigation of in vitro and in vivo antioxydant activities of flavonoids rich extract from the berries of Rhodomyrtus tomentosa (Ait.) Hassk. Food Chem., 173: 194–202.