Study on characterization of chalcone synthase gene from \(\textit{Pueraria lobata}\) and \(\textit{Pueraria mirifica}\) in Vietnam


  • Huynh Thi Thu Hue 1)Institute of Genome Research, Vietnam Academy of Science and Technology2) Graduate University of Science and Technology, Vietnam Academy of Science and Technology
  • Nguyen Minh Phuong 3)Vietnam National University of Agriculture
  • Nguyen Xuan Canh 3)Vietnam National University of Agriculture



Chalcone synthase, CHS, gene analysis, P.lobata, P.mirifica.


Two species of genus Pueraria ((Pueraria lobata (synonym: Pueraria montana var. lobata) and Pueraria mirifica (synonym: Pueraria candollei var. mirifica)) are traditional plants used in medicine since ancient times. These plants have been used and became commercially crucial indigenous medicinal plants. Currently, both roots and flowers of P. mirifica are used as a dietary supplement and functional food for women because of their rich source of phytoestrogen and nutrition. However, little information of genes on both species of Pueraria genus (P. lobata and P. mirifica) are known in Vietnam. The purpose of this research is to support more understanding about Chalcone synthase (CHS) genes by determining and sequence analyzing an encoding region of CHS genes that were isolated from P. lobata and P. mirifica. The full-length open reading frame (ORF) sequence CHS was identified with 1170 bp which encodes 389 amino acids by Sanger sequencing. The isolated CHS gene of P. lobata has no difference in sequence with CHS reported on GenBank (D10223.1), whereas a difference of 26 nucleotide positions in CHS sequence of P. mirifica compared with the published gene sequence (JQ409456.1) as consequential having  97.78% genetic similarity. The CHS genes sequence of P. lobata and P. mirifica are homologous with 98.4% because of having 19 nucleotide differences. Chalcone-stilbene synthase N-C terminal, PLN03173, CHS-like, BH0617, fabH are some important domains predicting the CHS genes. Especially, the family signature ‘GVLFGFGPGLTI’ motif of CHS gene as a part of the active-site scaffold contributes to decide the product of cyclization reactions performing the stereochemistry of cyclization which was also observed in P. lobata and P. mirifica, but it was not included for all members in Fabaceae family. With in sillico analysis, the P. lobata and P. mirifica CHS sequences have highly conserved regions to maintain their structure and function, so that it needs further studies to clarify these points.


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Austin M. B., Noel J. P., 2003. The chalcone synthase superfamily of type III polyketide synthases. Nat. Pro. Rep., 20(1): 79−110.

Birt D. F., Hendrich S., Wang W., 2001. Dietary agents in cancer prevention: flavonoids and isoflavonoids. Pharmaco Ther, 90(2-3): 157−177.

Cherdshewasart W., Subtang S., Dahlan W., 2007. Major isoflavonoid contents of the phytoestrogen rich-herb Pueraria mirifica in comparison with Pueraria lobata. J Pharm .Biomed. Anal ., 43(2): 428−434.

Cheung D. W. S., Koon C. M., Ng C.F., Leung P. C., Fung K. P., Poon S. K. S., Lau C. B. S., 2012. The roots of Salvia miltiorrhiza (Danshen) and Pueraria lobata (Gegen) inhibit atherogenic events: a study of the combination effects of the 2-herb formula. J. Ethnopharmacol., 143(3): 859−866.

Claudot A. C., Ernst D., Sandermann H., Drouet A., 1999. Cloning and characterization of two members of the chalcone synthase gene family from walnut. Plant. Physiol. Biochem., 37(10): 721−730.

Dao T.T. H., Linthorst H. J. M., Verpoorte R., 2011. Chalcone synthase and its functions in plant resistance. Phytochem. Rev., 10(3): 397−412.

Dewick, P. M., 2009. The mevalonate and methylerythritol phosphate pathways: terpenoids and steroids. Medicinal natural products: a biosynthetic approach. UK, Wiley: 187-306.

Dixon R.A., Steele C. L., 1999. Flavonoids and isoflavonoids-a gold mine for metabolic engineering. Trends. Plant. Sci., 4(10): 394−400.

Estabrook E. M., Gopalan C. S., 1991. Differential Expression of Phenylalanine Ammonia-Lyase and Chalcone Synthase during Soybean Nodule Development. Plant. Cell., 3(3): 299−308.

Ferrer J. L., Jez J. M., Bowman M. E., Dixon R. A., Noel J. P., 1999. Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis. Nat. Struct. Biol., 6(8): 775−783.

Healthstore. FSC Kudzu Capsule, 2020. Available from: Accessed on 28 November 2020.

Hrazdina G., Lifson E., Weeden N. F., 1986. Isolation and characterization of buckwheat (Fagopyrum esculentum M.) chalcone synthase and its polyclonal antibodies. Arch. Biochem. Biophys., 247(2): 414−419.

Ichinose Y., Kawamata S., Yamada T., An C. C., Kajiwara T., Shiraishi T., Oku H., 1992. Molecular cloning of chalcone synthase cDNAs from Pisum sativum. Plant. Mol. Biol., 18(5): 1009−1012.

Iwasaki M., Inoue M., Otani T., Sasazuki S., Kurashi N., Miura T., Yamoto S., Tsugane S., 2008. Plasma isoflavone level and subsequent risk of breast cancer among Japanese women: a nested case-control study from Japan Public Health Center-base prospective study group. J. Clin. Oncol., 26(10): 1677−1683.

Keung W. M., Vallee B. L., 1998. Kudzu root: an ancient Chinese source of modern antidipsotropic agents. Phytochemistry 47(4): 499-506.

Manonai J., Chittacharoen A., Theppisai U., Theppisai H., 2007. Effect of Pueraria mirifica on vaginal health. Menopause 14(5): 919−924.

Miadokova E., Masterova I., Vlckova V., Duhova V., Toth J., 2002. Antimutagenic potential of homoisofl avonoids from Muscari racemosum. J. Ethnopharmacol ., 81(3): 381−386.

Nakajima O., Akiyama T., Hakamatsuka T., Shibuya M., Noguchi H., Ebizuka Y., Sankawa U., 1991. Isolation, sequence and bacterial expression of a cDNA for chalcone synthase from the cultured cells of Pueraria lobata. Chem. Pharm. Bull ., 39(7): 1911−1913.

Nakajima O., Shibuya M., Hakamatsuka T., Noguchi H., Ebizuka Y., Sankawa U., 1996. cDNA and Genomic DNA Clonings of Chalcone Synthase from Pueraria lobata. Biol. Pharm. Bull., 19(1): 71−76.

Pandith S. A., Ramazan S., Khan M. I., Reshi Z. A., Shah M. A., 2019. Chalcone synthases (CHSs): the symbolic type III polyketide synthases. Planta, 251(1):15

Rohde W., Dörr S., Salamini F., Becker D., 1991. Structure of a chalcone synthase gene from Hordeum vulgare. Plant. Mol. Biol., 16(6): 1103−1106.

Ryan- Borchers T. A., Park J. S., Chew B. P., McGuire M. K., Fournier L. R., Beerman K. A., 2006. Soy isoflavones modulate immune function in healthy postmenopausal women. Am. J .Clin .Nutr., 83(5): 1118−1125.

Ryder T. B., Hedrick S. A., Bell J. N., Liang X. W., Clouse S. D., Lamb. J., 1987. Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vulgaris. Mol. Gen. Genet., 210(2): 219−233.

Sambrook J., Russell D. W., 2001. Molecular Cloning. A Laboratory Manual, 3rd ed. Cold.

Scarpato R., Paganucci L., Bertoli A., Fiore L., Pistelli L., Federico G., 2008. Licoflavone C attenuates the genotoxicity of cancer drugs in human peripheral lymphocytes. Phytother. Res., 22(12): 1650−1654.

Shao L., Li Y., Pan A., Cheng Z., Chen M., 1995. Molecular cloning, sequencing, and expression in Escherichia coli of the chalcone synthase gene. Chin. J. Biotechnol., 11(2): 131−135.

Suh D. Y., Fukuma K., Kagami J., Yamazaki Y., Shibuya M., Ebizuka Y., Sankawa U., 2000. Identification of amino acid residues important in the cyclization reactions of chalcone and stilbene synthases. Biochem. J., 350(Pt 1): 229−235.

TGA. Australian Register of Therapeutic Goods Medicine. 2020; Available from: Accessed on 28 November 2020.

Wanadorn P. W., 1933. A reputed rejuvenator. J. Siam. Soc. Nat. Hist., 9(1): 145−147.

Wani T. A., Pandith S. A., Gupta A. P., Chandra S., Sharma N., Lattoo S. K., 2017. Molecular and functional

characterization of two isoforms of chalcone synthase and their expression analysis in relation to flavonoid constituents in Grewia asiatica L. PloS One, 12(6): e0179155. 10.1371/journal.pone.0179155

Wiriyaampaiwong P., Thanonkeo P., Thanonkeo S., 2012. Cloning and Characterization of Chalcone Synthase Gene from Pueraria candollei var. mirifica. J. Med. Plans. Res., 6(42): 5469−5479.




How to Cite

Thu Hue, H. T. ., Phuong, N. M., & Canh, N. X. (2021). Study on characterization of chalcone synthase gene from \(\textit{Pueraria lobata}\) and \(\textit{Pueraria mirifica}\) in Vietnam. Academia Journal of Biology, 43(3), 47–58.