Creation in vitro materials and initial study of creating hairy root from Plumbago zeylanica L

Authors

  • Bui Dinh Thach Institute of Tropical Biology, VAST
  • Nguyen Minh Hieu Institute of Tropical Biology, VAST
  • Pham Thi Phuong Uyen Institute of Tropical Biology, VAST
  • Ngo Ke Suong Institute of Tropical Biology, VAST
  • Nguyen Huu Ho Institute of Tropical Biology, VAST

DOI:

https://doi.org/10.15625/0866-7160/v34n3se.1796

Keywords:

Agrobacterium rhizogen, Plumbago zeylanica, hairy root, in vitro

Abstract

Plumbago zeylanica L. is a medicinal plant that contains high levels of the active ingredient plumbgin. With the goal of creating raw materials in vitro, 20-minute disinfection time, 10% solution is the optimum level to create templates in vitro (41.29%), MS medium supplemented with 1.5 mg/L BA and 0.1 mg L IBA create optimal shoots (4.67 shoots/sample) after 4 weeks of culture. In vitro shoots developed into the best tree in each MS supplemented with 0.1 mg / L IBA. Two strains of Agrobacterium rhizogen ATCC-15834 and ATCC-11325 used for transgenic stimulation ofhairy roots from in vitro leaves. The initial results have been selected for hairy root 5 lines.

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Published

06-08-2012

How to Cite

Thach, B. D., Hieu, N. M., Phuong Uyen, P. T., Suong, N. K., & Ho, N. H. (2012). Creation in vitro materials and initial study of creating hairy root from Plumbago zeylanica L. Academia Journal of Biology, 34(3se), 161–169. https://doi.org/10.15625/0866-7160/v34n3se.1796

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