Evaluation of CYP11Bs expression in HCT116 cells
Keywords:Bovin Adrenodoxin (bAdx), COS-1 cell, CYP11B1, CYP11B2, HCT116 cell, Human Adrenodoxin (hAdx).
CYP11B1 and CYP11B2 are enzymes respectlively responsible for cortisol and aldosterone biosynthesis. In human steroid biosynthesis, CYP11B1 converts 11-deoxycortisol to cortisol and CYP11B2 converts 11-deoxycorticosterone to aldosterone. In this study, we utilized methods cell culture (COS-1 and HCT116), transfection of vector pSVL, pSVL+11B1, pSVL+11B2 and coexpression of bAdx/hAdx into 2 cell culture. Steroids were extracted by using chloroform and separated on high performance thin layer chromatography (HPTLC) on silica plate. Results showed evaluation of CYP11Bs in HCT116 cells. The effect of electron transport protein of bovin adrenodoxin was better than electron transport protein of human adrenodoxin in HCT116 cells. Therefore, HCT116 cell could be used to study the metabolism of steroids by 2 isozyme CYP11Bs.