Research on neuroprotective effects of a fusion protein of omega-conotoxin mviia and thioredoxin

Authors

  • Bui Thi Huyen VAST
  • Doan Viet Binh VAST
  • Nguyen Thi Kim Dung VAST
  • Le Thi Bich Thao VAST
  • Nguyen Bich Nhi VAST
  • Phan Van Chi VAST

DOI:

https://doi.org/10.15625/0866-7160/v34n4.2694

Keywords:

conotoxin, ischemia, neuroprotectiv, omega-conotoxin MVIIA, Trx-CTX

Abstract

Ischemic stroke is one of the most dangerous human diseases. Blood supply to the brain is decreased initiating the ischemic cascade with the consequence of a Ca2+ influx into nerve-cells. This Ca2+ influx can than cause neuronal injury or death of cells. Omega-conotoxin MVIIA (omega-CTX) is a neurotoxin isolated from the venom of Cone snail Conus magus. It is a potent selective blocker of the N-type voltage-sensitive calcium channel in neurons and therefore has the potential to promote neuroprotection through inhibition of Ca2+ influx into nerve-cells. Omega-CTX has been cloned, expressed in the fusion form with thioredoxin (Trx-CTX ) in E. coli in the institute of biotechnology, Hanoi. In this paper we present our study on neuroprotectiv activity of Trx-CTX in mouse model of global cerebral ischemia and focal brain ischemia. The experiments were carried out in male mice of Swiss race weighing 40 g. Trx-CTX were injected i.v. in the model of global cerebral ischemia, one time with a dose of 5 or 10 mg/kg and in the other model two times, each with a dose of 5 mg/kg. The results showed that in global cerebral ischemia Trx-CTX has decreased neuronal injury and death of CA1 cells of dorsal hippogampus. In other model Trx-CTX also decreased infarction size of the ischemic brain. Trx-CTX proof to possess neuroprotective effect and could be used in further researchs for medicinal purpose.

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Published

13-01-2013

How to Cite

Huyen, B. T., Binh, D. V., Kim Dung, N. T., Bich Thao, L. T., Nhi, N. B., & Chi, P. V. (2013). Research on neuroprotective effects of a fusion protein of omega-conotoxin mviia and thioredoxin. Academia Journal of Biology, 34(4), 515–520. https://doi.org/10.15625/0866-7160/v34n4.2694

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