Cloning Shrunken 2 (Sh2) gene from h14 maize cultivar and constructing transformation vector pcambia 1301-Ubi-Sh2

Authors

  • Nguyen Thi Thu Viện Công nghệ sinh học, Viện Hàn lâm KH & CN Việt Nam
  • Le Hoang Duc Viện Công nghệ sinh học, Viện Hàn lâm KH & CN Việt Nam
  • Nguyen Duc Thanh Viện Công nghệ sinh học, Viện Hàn lâm KH & CN Việt Nam

DOI:

https://doi.org/10.15625/0866-7160/v35n3se.3849

Keywords:

ADP-glucose pyrophosphorylase, cloning, H14 maize cultiva, Shrunken 2 gene, transformation vector

Abstract

 

The Shrunken 2 (Sh2) gene encodes the large subunit of endosperm ADP-glucose pyrophosphorylase – one of the enzymes that regulate the starch biosynthetic pathway. This gene had been transferred, successfully, to maize plant. The transgenic maize plants have higher starch content than that of wild type. In this article, we present the results on cloning the Sh2 gene by PCR from H14 maize cultivar and constructing transformation vector pCambia1301-Ubi-Sh2. The cloned Sh2 sequence had high similarity (99%) with sequence of Sh2 gene in Genbank with assesssion number NM_001127632.1. The sequence of Sh2 gene from H14 maize cultivar was submited to the GenBank with assession number JX462603. The gene was, then, successfully, inserted into expression vector pCambia1301 along with Ubiquitin promoter to construct a transformation vector pCambia 1301-Ubi-Sh2, that will be used for genetic engineering of starch in maize.

Downloads

Download data is not yet available.

Downloads

Published

03-04-2014

How to Cite

Thu, N. T., Duc, L. H., & Thanh, N. D. (2014). Cloning Shrunken 2 (Sh2) gene from h14 maize cultivar and constructing transformation vector pcambia 1301-Ubi-Sh2. Academia Journal of Biology, 35(3se), 122–128. https://doi.org/10.15625/0866-7160/v35n3se.3849

Issue

Section

Articles

Most read articles by the same author(s)

1 2 3 > >>