Enhancing expression of recombinant fimbrial adhesin K88 isolated of enterotoxigenic Escherichia coli from piglet

Authors

  • Nguyen Hoang Loc College of Sciences, Hue University
  • Nguyen Thi Khanh Quynh Thua Thien - Hue Department of Science and Technology
  • Hoang Tan Quang Institute of Biotechnology, Hue University
  • Tran Thuy Lan Institute of Biotechnology, Hue University
  • Le My Tieu Ngoc Institute of Biotechnology, Hue University
  • Dinh Thi Bich Lan Institute of Biotechnology, Hue University
  • Phung Thang Long College of Agriculture and Forestry, Hue University

DOI:

https://doi.org/10.15625/0866-7160/v36n1se.4378

Keywords:

Escherichia coli, enterotoxin, fimbrial adhesin, weaned piglet.

Abstract

Fimbrial adhesins are important toxic factors of enterotoxigenic E. coli (ETEC) strains, whereby they have the ability to adhere to small intestine of the host. K88 (F4) is the first fimbrial adhesin was detected in ETEC strains from piglet, and it is the most popular fimbrial adhesin of E. coli strains causes diarrhea in weaned piglets. In previous report, we successful expressed the faeG gene encoding fimbrial adhesin K88-1NT in recombinant E. coli BL21 (DE3). Highest level of K88-1NT was determined after induction by 0.1% lactose for 6 h at 35oC when cell density (OD600) reached a value of 0.5. Induction medium including Na2HPO4.12H2O 1%, KH2PO4 0.3%, NaCl 0.05%, (NH4)2SO4 1%, MgSO4.7H2O 0.2%, glucose 1.5%, tryptone 1% and yeast extract 1%. Purified K88-1NT protein from Ni2+ affinity chromatography had a molecular weight of 31 kDa.

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Published

06-08-2014

How to Cite

Loc, N. H., Khanh Quynh, N. T., Quang, H. T., Lan, T. T., Tieu Ngoc, L. M., Bich Lan, D. T., & Long, P. T. (2014). Enhancing expression of recombinant fimbrial adhesin K88 isolated of enterotoxigenic Escherichia coli from piglet. Academia Journal of Biology, 36(1se), 120–125. https://doi.org/10.15625/0866-7160/v36n1se.4378

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Section

Articles