The selection of the lactic acid bacteria forbiomass production in nursing striped catfish
Keywords:Enterococcus hirae, gain ratio, lactic bacteris, survival ratio.
The aim of this study is to select the lactic acid bacteria to produce biomass in nursing striped catfish. After isolation and antibiotic substrate testing, the genera Enterococcus hirae isolated from fermented fish product was the most promising. The 3 factorial experimental design was used for E. hirae biomass production. The results showed that the optimum dried biomass 0.85 g/l was obtained in supplements: succrose 11%, (NH4)2SO4 0.48% and KH2PO4 0.25%. The dried biomass was stored in 6 months at room temperature with the density of 108CFU/g. The E. hirae in dried biomass was revived in warm water at 40oC, for 30 minutes. The randomized complete experimental design was used for nursing of 30 Pangasianodon hypophthalmus fingerlings by Aquaxcel-40N in 28 days, with or without E. hirae solution: 0 ml (control=treatment No1 ), 30 ml (treatment No2), 50 ml (treatment No3). The results showed that the survival ratio of P. hypophthalmus fingerlings increased 38% on No3 treatment, significant different in comparison with control, the everage weight gain of P. hypophthalmus fingerlings was also increased 13% on No3 treatment and 7.8% on No 2 treatment, significant different in comparison with control. In conclusion that, E. hirae supplementation for nursing of Pangasianodon hypophthalmus fingerlings in order to increase the survival ratio and weight gain.