Optimization of medium composition for recombinant nattokinase production from Bacillus subtillis by using response surface methodology

Authors

  • Tran Quoc Tuan University of Sciences, National University of Ho Chi Minh city
  • Nguyen Thi Thu Kieu University of Sciences, National University of Ho Chi Minh city
  • Le Thi Thuy Ai University of Sciences, National University of Ho Chi Minh city
  • Dinh Minh Hiep Department of Science and Technology Ho Chi Minh city
  • Tran Cat Dong Faculty of Pharmacy - Medical University of Ho Chi Minh city

DOI:

https://doi.org/10.15625/0866-7160/v36n1se.4380

Keywords:

Bacillus subtilis, fibrinolytic activity, nattokinase activity, recombinant nattokinase, response surface methodology.

Abstract

Nattokinase is a serine protease with fibrinolytic activity whose clinical efficacy and safety for human by the oral route has been proven. In this study, we optimized the medium components for production of recombinant nattokinase from Bacillus subtillis DB104. Carbon and nitrogen sources from the different materials were selected in order to make the base for screening factors affecting nattokinase activity by Plackett-Burman experiments. Among factors surveyed, soya pepton, MgSO4 and NaCl were considered significant (p<0.05). These factors were subsequently optimized by using the response surface methodology (RSM). The obtained results showed the appropriate mediumfor the biosynthesis of nattokinase included 10 g/l soypepton, 0.88 g/l MgSO4, 5 g/l NaCl. After 16 hrs of fermentation, nattokinase activity reached the highest level of 152 FU/ml, 1.8 times higher than that in the pre-optimized one (83 FU/ml).

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Published

06-08-2014

How to Cite

Tuan, T. Q., Thu Kieu, N. T., Thuy Ai, L. T., Hiep, D. M., & Dong, T. C. (2014). Optimization of medium composition for recombinant nattokinase production from Bacillus subtillis by using response surface methodology. Academia Journal of Biology, 36(1se), 130–137. https://doi.org/10.15625/0866-7160/v36n1se.4380

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