Study on the ability of extracts from Cordyceps spp. biomass to prevent memory impairment in mice
Keywords:Cordyceps, acetylcholinesterase, galantamine, memory impairment, trimethyltin.
Following screening acetylcholinesterase (AChE) inhibitory activity of 60 extracts from biomass of 10 Cordyceps spp. strains, 3 extracts with high AChE inhibitor activity, viz. polysaccharide DL0004 extract (51.80±1.41%), n-BuOH DL0006 extract (55.71±2.72%) and n-BuOH DL0015 extract (71.08±1.47%) were further tested for their ability to improve memory impairment in mice using two short-term memory impairment models: Y-maze model (Y-maze) and Novel Object Recognition model. Cordyceps was extracted at a dose of 100 mg/kg and 200 mg/kg three days before the intraperitoneal (i.p) injection of 2.4 mg/kg trimethyltin (TMT); and seven days later, the mice were tested on the models. Results on the Y-maze model showed that there was a statistically significant difference in combinatorial percentage among different combinations: DL0004.1 and TMT (p<0.01), TMT and DL0004.2 (p<0.05), TMT and DL0015.1 (p<0.01), TMT and DL0006.1 (p<0.05), TMT and DL0006.2 (p<0.05). For Novel Object Recognition model, the results also showed difference with statistical significance in the ratio of novel recognition to familiar recognition among comparison combinations: TMT and DL0004.1 (p<0.001), TMT and DL0004.2 (p<0.05), TMT and DL0006.2 (p<0.05), TMT and DL0015.1 (p<0.001).