Cloning and sequencing of gene coding antigen of lung cancer, cyfra 21-1

Authors

  • Le Dinh Chac VAST
  • La Thi Huyen
  • Le Quang Huan

DOI:

https://doi.org/10.15625/0866-7160/v30n3.5439

Abstract

Cytokeratin 19 (CK19), with a molecular weight of 40 kDa, is the specific cytoskeletal structure of simple epithelia. CK19 has been used as a marker of circulating epithelial cells to diagnose cancer metastasis since it is probably one of the most suitable markers for cancer. One of the fragments of CK19, CYFRA 21-1, has been introduced as a tumor marker for primary lung cancer and proven suitable for the diagnosis of squamous cell carcinoma of the lung.

We have amplified the gene encoding for CYFRA 21-1 from the lung cancer samples by RT-PCR technology with the specific primers. The gene sequence has a length of 1001 bp and its homology with the gene sequence CYFRA 21.1 (accession number AB041270) from the GenBank data is 99.82%. This sequence has one point mutation G766C when compared with CYFRA 21-1 gene sequence from the GeneBank.

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Published

13-11-2014

How to Cite

Chac, L. D., Huyen, L. T., & Huan, L. Q. (2014). Cloning and sequencing of gene coding antigen of lung cancer, cyfra 21-1. Academia Journal of Biology, 30(3), 165–168. https://doi.org/10.15625/0866-7160/v30n3.5439

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Articles