Activity test of antibody recombinant specificity Escherichia coli O157:H7

Authors

  • Hoang Phu Hiep VAST
  • Le Thi Kim Xuan
  • Nguyen Minh Lua
  • Nguyen Thi Minh Huyen
  • Tran Manh Ha
  • Dao Minh Duc
  • Nguyen Bich Nhi
  • Le Quang Huan

DOI:

https://doi.org/10.15625/0866-7160/v36n3.5975

Keywords:

E. coli O157, H7, pET28a TRX, recombinant antibodies, nano silica

Abstract

Antibodies are immunoglobulin molecules which originally are glycoprotein that play role to identify and neutralize foreign agents such as viruses or bacteria.

In this study, antibody protein recombine specificity of E. coli O157:H7 which was purified using hybrid methods. Next, antibody activity was tested by the methods as Dot Blot, Western Blot & ELISA. The results showed thatantibody protein specificly paired with E. coli O157:H7. Initially determine the specificity of antibodies by recombinant methods silica nanoparticles attached to antibodies and staining with E. coli O157:H7 and examined under a microscope. Thereby creating a scientific basis for the study of microbial diagnostic kit created in food samples as well as providing additional sources of antibody therapy of poisoning caused by bacteria E. coli O157:H7.

Downloads

Download data is not yet available.

Downloads

Published

19-03-2015

How to Cite

Hiep, H. P., Kim Xuan, L. T., Lua, N. M., Minh Huyen, N. T., Ha, T. M., Duc, D. M., Nhi, N. B., & Huan, L. Q. (2015). Activity test of antibody recombinant specificity Escherichia coli O157:H7. Academia Journal of Biology, 36(3), 340–344. https://doi.org/10.15625/0866-7160/v36n3.5975

Issue

Section

Articles

Most read articles by the same author(s)

1 2 > >>