Medium optimization for biosurfactant production by Rhodococcus ruber TD2 using response surface methodology

Authors

  • Vuong Thi Nga VAST
  • Kieu Quynh Hoa
  • Tran Dinh Man
  • Lai Thuy Hien

DOI:

https://doi.org/10.15625/0866-7160/v36n3.5976

Keywords:

Rhodococcus, biosurfactant, response surface methodology, medium optimization

Abstract

With the advanced properties such as excellent emulsifying activity, reducing surface tension, antitumor and immunomodulation, Rhodococcus biosurfactants have attracted a great deal of interest from biologists community. Development of optimized procedures for their biosynthesis would increased application potentials of Rhodococcus biosurfactants in new advanced technologies, such as environmental bioremediation, biomedicine, etc. The goal of this study is to determine the medium optimization for biosurfactant production by Rhodococcus ruber TD2 using  response surface methodology (RSM) based on changes of medium component: diesel oil (DO), NaNO3 and pH. All of three factors were the most significant influences on its biosurfactant biosynthesis. RSM analysis showed that the highest biosurfactant production was achieved with medium containing 5.7% (v/v) DO, 3.3 gL-1 NaNO3 and pH 8.3. In this condition, strain TD2 produced maximum yield of crude biosurfactant, which was 30.1 gL-1 after 5 days. This value was 2.23- fold higher than before medium optimization. The data showed that RSM was highly efficient in optimizing biosurfactant  production by Rhodococcus ruber TD2 isolated from coastal zone of Vietnam.

Downloads

Download data is not yet available.

Downloads

Published

19-03-2015

How to Cite

Nga, V. T., Hoa, K. Q., Man, T. D., & Hien, L. T. (2015). Medium optimization for biosurfactant production by Rhodococcus ruber TD2 using response surface methodology. Academia Journal of Biology, 36(3), 360–366. https://doi.org/10.15625/0866-7160/v36n3.5976

Issue

Section

Articles

Most read articles by the same author(s)

1 2 > >>