Conditions of limited hydrolysis producing pectic oligosaccharides (pos) of high concentration passion fruit pectin
Keywords:Limited hydrolysis, passion fruit pectin, pectic oligosaccharide, pectinase, prebiotic.
Pectic oligosaccharides (POS) have been proposed as a new class prebiotics capable of exerting a number of health-promoting effects. POS can be obtained by limited hydrolysis enzyme of pectin. The present study investigates the conditions of limited hydrolysis passion fruit pectin (Passiflora edulis flavicarpa) by pectinase. Pectin with concentration 1-5% (w/v) was hydrolyzed by Pectinex Ultra SP-L 30-50 U/g, pH 3-5, 40-60°C at 200-350 rpm with hydrolysis time of 1-7 hours. Research results show that pectin 3% (w/v), 40 U/g, pH 4, 50oC, 250 rpm, products have 18.76 mg POS/ml. Response surface methodology was used to model the processes. The temperature (45-55°C), enzyme concentration (30-50 U/g), and speed agitation (200-300 rpm) were the factors studied. Operational conditions leading to a maximum POS production were calculated from models being 53oC, 44 U/g, 270 rpm. Under the conditions, the model predicted that 24.55 mg/ml of POS can be obtained from pectin. Conversions to product increased from 62,53 to 81,83%. Products contain di and tri galacturonic acid.