Heterologous expression of cis-prenyltransferase 1 for production of neryl pyrophosphate in Escherichia coli

Authors

  • La Ngoc Thuy Van VAST
  • Le Thi Kim Lan
  • Khuat Le Uyen Vy
  • Dinh Minh Hiep
  • Eran Pichersky
  • Nguyen Thi Hong Thuong

DOI:

https://doi.org/10.15625/0866-7160/v37n1se.6114

Keywords:

cis-prenyltransferase, neryl pyrophosphate, monoterpene synthase

Abstract

Neryl pyrophosphate (NPP), also known as neryl diphosphate (NDP), is the cis isomer of genaryl pyrophosphate (GPP). Previously, it has been shown that monoterpene synthases found in plants use GPP as a substrate for the synthesis of monoterpenes and monoterpenoids. However, in 2009, Schilmiller identified a new monoterpene synthase gene for phellandrene synthase (PHS) that is expressed in glandular trichomes of the cultivated tomato Solanum lycopersicum and uses NPP as a substrate to produce β-phellandrene as a main product. An mRNA for a cis-prenyltransferase (cis-prenyltransferase 1, CPT1) was also identified in these glands. It encodes an enzyme that uses DMAPP and IPP to produce NPP. E. coli has the ability to synthesize GPP but not NPP. In this study, we cloned and expressed CPT1 in E. coli for production of NPP followed by coupling with a monoterpene synthase such as PHS, which specifically uses NPP as a substrate to produce β-phelandrene. Further metabolic engineering of the pathway and in situ product recovery would make this engineered E. coli a potential production platform for many valuable monoterpenes and their derivatives.

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Published

25-04-2015

How to Cite

Thuy Van, L. N., Kim Lan, L. T., Uyen Vy, K. L., Hiep, D. M., Pichersky, E., & Hong Thuong, N. T. (2015). Heterologous expression of cis-prenyltransferase 1 for production of neryl pyrophosphate in Escherichia coli. Academia Journal of Biology, 37(1se), 224–230. https://doi.org/10.15625/0866-7160/v37n1se.6114

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