Rapid micropropagation of Begonia tuberous by young leaf tissue culture and acclimatization in greenhouse

Authors

  • Dinh Van Khiem VAST
  • Le Thi Nhu Lan
  • Duong Tan Nhut
  • Mai Xuan Luong

DOI:

https://doi.org/10.15625/0866-7160/v26n3.6904

Abstract

In this paper, the protocol for the rapid micropropagation of Begonia tuberous are presented.

The shoot regeneration was carried out on 1/2MS medium, plus 0.7 mg/l BA and 0.03 mg/l NAA. Furthermore, the callus formation was obtained on 1/2MS medium supplemented with 0.7 mg/l BA and 0.1 mg/l NAA.

For the shoot multiplication, single shoots were cultured on MS medium, plus 1 mg/l BA and 0.3 mg/l NAA.

The root formation was obtained on 1/2MS medium, plus 0.5 mg/l NAA and 0.3  mg/l IBA.

To increase the growth and development possibility of Begonia tuberous in the greenhouse, we can use some chemical fertilizers (nitrophoska, Grow more, super) at a suitable dosage.

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Published

05-09-2015

How to Cite

Khiem, D. V., Nhu Lan, L. T., Nhut, D. T., & Luong, M. X. (2015). Rapid micropropagation of Begonia tuberous by young leaf tissue culture and acclimatization in greenhouse. Academia Journal of Biology, 26(3), 56–63. https://doi.org/10.15625/0866-7160/v26n3.6904