Micropropagation of Dendrobium sp. via somatic embryogenesis culture

Authors

  • Bui Thi Tuong Thu
  • Tran Van Minh

DOI:

https://doi.org/10.15625/0866-7160/v33n4.785

Abstract

Protocorm like bodies were used as planting materials. PLBs were cut into slices and placed on the medium for callus initiated. Callus was initiated on the medium MS + CW (30%) + 2.4D (0.5 mg/l). Callus proliferates on the medium MS + CW (10%) supplemented with NAA (0.5 mg/l) + 2.4D (0.1 mg/l). Callus was regenerated on the medium MS + (10%) supplemented with + NAA (0.1 mg/l) + BA (0.1 mg/l). Somatic cell suspension was initiated on MS + 2.4D (0.5 mg/l) and proliferated on the medium MS + CW (10%) supplemented was NAA (0.5 mg/l) + 2.4D (0.1 mg/l). Somatic cell suspension was differentiated to embryogenic cell suspension on the medium MS +  CW (10%) supplemented with NAA (0.1 mg/l) + BA (0.1 mg/l). Embryogenic cell suspension was plating and regeneration on the medium MS + CW (10%) + NAA (0.1 mg/l) + BA (0.1 mg/l).

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Published

09-05-2012

How to Cite

Tuong Thu, B. T., & Minh, T. V. (2012). Micropropagation of Dendrobium sp. via somatic embryogenesis culture. Academia Journal of Biology, 33(4), 72–78. https://doi.org/10.15625/0866-7160/v33n4.785

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Section

Articles