Selection of fermentation condition for expression of recombinant single chain antibody recognizing the antigen of blood type a in Escherichia coli

Authors

  • Dang Thi Ngoc Ha Viện Công nghệ Sinh học
  • Nguyen Thi Trung
  • Le Thi Thu Hong Viện Công nghệ sinh học
  • Do Thi Huyen Viện Công nghệ sinh học
  • Truong Nam Hai Viện Công nghệ sinh học

DOI:

https://doi.org/10.15625/0866-7160/v39n2.8485

Keywords:

Escherichia coli, blood group A antigen, single chain recombinant antibody

Abstract

Single chain recombinant antibody identifying blood group A antigen in the ABO system (antiA_scFv) was constructed to express in E. coli. In this study, we optimized fermentation conditions to increase the yield of antiA_scFv expression as well as to reduce cost and save culture time. The results showed that the antiA_scFv expression was highest in the culture TB medium with 0.1 mM IPTG, at 30oC. Induction time point was in exponential growth phase of cells at the cell density OD600 = 1.5. With the selected fermentation conditions, the cell biomass increased 5.2 times (from OD600 = 3.27 to OD600 = 17.02) and antiA_scFv yield increased significantly as compared to the initial conditions.

 

Citation: Dang Thi Ngoc Ha, Le Thi Thu Hong, Do Thi Huyen, Truong Nam Hai, 2017. selection of fermentation condition for expression of recombinant single chain antibody recognizing the antigen of blood type a in escherichia coli. Tap chi Sinh hoc, 39(2): 204-211. DOI: 10.15625/0866-7160/v39n2.8485

*Corresponding author: tnhai@ibt.ac.vn

Received 11 July 2016, accepted 20 March 2017

 

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Author Biographies

Dang Thi Ngoc Ha, Viện Công nghệ Sinh học

Phòng Kỹ thuật di truyền

Le Thi Thu Hong, Viện Công nghệ sinh học

Phòng Kỹ thuật Di truyền

Do Thi Huyen, Viện Công nghệ sinh học

Phòng Kỹ thuật Di truyền

Truong Nam Hai, Viện Công nghệ sinh học

Phòng Kỹ thuật Di truyền

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Published

24-03-2017

How to Cite

Ngoc Ha, D. T., Trung, N. T., Thu Hong, L. T., Huyen, D. T., & Hai, T. N. (2017). Selection of fermentation condition for expression of recombinant single chain antibody recognizing the antigen of blood type a in Escherichia coli. Academia Journal of Biology, 39(2), 191–198. https://doi.org/10.15625/0866-7160/v39n2.8485

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