Cuture fibroblasts isolated from mouse tissues

Authors

  • Tran Thi Khanh Hoa Trung tâm Nghiên cứu Triển khai - Khu Công nghệ cao Thành phố Hồ Chí Minh
  • Hoang Thuy Duong
  • Nguyen Thi Kim Anh

DOI:

https://doi.org/10.15625/0866-7160/v40n1.8789

Keywords:

Fibroblast, kidney, lung, subculture, tail bone

Abstract

Fibroblasts are cells which multiple a thounsand times or cultured from the different tissue types with special conditions for each case to obtain the optimal number of cells. This study aims to compare the growth of primary cultured fibroblast cells derived from different tissues of mice and the influence of the replay subculture on cell’s properties, which produce a large sources for biomedical researches. Primary cells were subcultured to maintain cell survival and proliferation. The development ability and cell survival were tested by means of cell counting using Trypan Blue and shapes are observed by 4',6-diamidino-2-phenylindole (DAPI) staining. From the experimental results, cells from the organ are capable of heterogeneous development in a certain time period; simultaneously, to obtain fibroblasts from each tissue type also requires different conditions. Also, study on the influence of the subcultures help to maintain cell life for future experiments.

 

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Author Biography

Tran Thi Khanh Hoa, Trung tâm Nghiên cứu Triển khai - Khu Công nghệ cao Thành phố Hồ Chí Minh

Phòng Công nghệ Sinh học

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Published

25-01-2018

How to Cite

Khanh Hoa, T. T., Duong, H. T., & Kim Anh, N. T. (2018). Cuture fibroblasts isolated from mouse tissues. Academia Journal of Biology, 40(1), 69–75. https://doi.org/10.15625/0866-7160/v40n1.8789

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Section

Articles