Transient expression of interleukin-7 in nicotiana benthamiana using agro-infiltration

Authors

  • Nguyen Huy Hoang Thai Nguyen University of Medicine and Pharmacy
  • Pham Bich Ngoc
  • Le Van Son
  • Chu Hoang Ha

DOI:

https://doi.org/10.15625/0866-7160/v39n2.9000

Keywords:

Nicotiana benthamiana, agro-infiltration, CD4, CD8, immune, IL-7, transient expression

Abstract

Interleukin-7 (IL-7) is a cytokine that plays an important role in promoting growth and development of CD4 and CD8 immune cells in humans. IL-7 is also a target of the attack of some invasive pathogens. In this study, IL-7- coding gene was cloned into pRTRA vector to generate a cassette 35S-IL7-cmyc-histag-100xELP, and then this cassette was inserted into a binary vector pCB301. This construct was transformed into Nicotiana benthamiana using agro-infiltration for transient expression assay. Western blot was used to confirm expression of IL-7 in N. benthamiana on the 5th day of post-agro-infiltration. The results show that there were two bigger size of IL-7 with molecular weights of 64 and 130 KDa resulted from post translational modification. IL-7 was purificated from 1 kg of fresh tobacco leaves with the quantity of 3.15% of total soluble protein using immobilized metal ion chromatography method and evaluated by the Image J software. These results set forward the stage of studies on the safety and therapeutic applications of plant-derived recombinant IL-7 protein to humans.

 

Citation: Nguyen Huy Hoang, Pham Bich Ngoc, Le Van Son, Chu Hoang Ha, 2017. Transient expression of a taste-modifying protein, interleukin-7, in nicotiana benthamiana using agro-infiltration. Tap chi Sinh hoc, 39(2): 232-238. DOI: 10.15625/0866-7160/v39n2.9000.

*Corresponding author: chuhoangha@ibt.ac.vn

Received 13 December 2016, accepted 20 May 2017

 

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Published

20-06-2017

How to Cite

Hoang, N. H., Ngoc, P. B., Son, L. V., & Ha, C. H. (2017). Transient expression of interleukin-7 in nicotiana benthamiana using agro-infiltration. Academia Journal of Biology, 39(2), 219–225. https://doi.org/10.15625/0866-7160/v39n2.9000

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