EVALUATION OF GENETIC DIVERSITY AND RELATIONSHIP OF INTRODUCED Santalum album L. PROVENANCES IN VIETNAM USING ISSR MARKERS
DOI:
https://doi.org/10.70169/VJFS.1022Keywords:
DNA markers, genetic diversity, ISSR markers, Santalum album LAbstract
The best five ISSR primers, selected from the screening of 20 primers, were used to evaluate the genetic diversity of 120 samples of Santalum album L. belonging to 8 seed sources/provenances collected at the plantations in Hoa Binh, Quang Ninh and Bac Giang provinces. The results obtained were 62 ISSR-PCR fragments, including 41.73% polymorphic fragments. Santalum album L. samples from 8 seedlots have low levels of diversity transmission with average values such as Na = 1.417; Ne = 1.225; I = 0.223; h = 0.149 and P = 41.73%. AMOVA analysis of the maximum level of transmission bias between provenances at a relatively high level reached 49% and the level of bias between samples within the same provenance reached 51% (PhiPT = 0.509). The genetic relationship tree combined with the STRUCTURE population structure has been divided into 3 groups, with group I (AnDo1, AnDo2, AnDo3 and VNam1 originating from India, group II originating from Indonesia (InĐo1 and InĐo2) and Group III (VNam2 and AnDo4). This result provides foundation knowledge for future research and development programs of Santalum album L. in Vietnam.
Keywords: DNA markers, genetic diversity, ISSR, Santalum album L.
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